文章摘要
北细辛内生真菌的分离鉴定及代谢产物的生物活性
Isolation,Identification and Metabolite Bioactivity of Endophytic Fungi from Asarum heterotropoides var. mandshuricum.
  
DOI：
中文关键词: 北细辛  内生真菌  分离  抗肿瘤活性  抗菌活性
英文关键词: Asarum heterotropoides  endophytic fungi  isolation  antitumor activities  antibacterial activities  
基金项目:国家重大新药创制专项
作者单位
包丽霞,殷瑜,杨天,杨和行,钱秀萍  
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中文摘要:
      采用表面消毒法分别从3种北细辛中分离获得10株形态特征不同的优势内生真菌,经形态学和18S rDNA ITS分子分类学分析鉴定为小丛壳属（Glomerella sp.）、叶点霉属（Phyllosticta sp.）、柄孢壳菌属（Po-dospora sp.）、刺盘孢属（Colletotrichum sp.）和镰孢属（Fusarium sp.）。对北细辛优势内生真菌的发酵产物进行体外抗肿瘤和抗菌活性检测,结果表明：除菌株E3、E4和E10外,其余菌株均有不同程度的抗肿瘤和抗菌活性;镰孢属（Fusarium sp.）菌株E9对A549、MDA-MB-231和PANC-1肿瘤细胞抑制率达75%以上;小丛壳属（Glomerella sp.）菌株E1和叶点霉属（Phyllosticta sp.）菌株E2靶向FabI的抗菌活性较强,抑制率达59%。
英文摘要:
      10 dominant endophytic fungal strains of different characteristics morphologically were isolated from three kinds of Asarum heterotropoides by the surface-sterilized method,and they were identified as Glomerella sp.,Phyllosticta sp.,Podospora sp.,Colletotrichum sp.and Fusarium sp.by morphology,18S rDNA internal transcribed spacer(ITS) molecular taxological analyses.The antitumor and antibacterial activities of their metabolites of these predominant endophytic fungi were detected in vitro.The results indicat...
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