手性拆分环氧氯丙烷菌株的筛选、鉴定及产酶条件研究

从土壤中筛选到5株环氧化物水解酶生产菌，并通过ITS序列鉴定了其中的C375菌，结果为黑曲霉（Aspergillus nigerZJB-09103）。考察了培养基不同碳源、氮源、金属离子和pH等对产酶的影响，得到了较佳的培养基条件：淀粉16g／L，豆饼粉3g／L，蛋白胨3g／L，KH2PO4 0．4g／L,K2HPO4 0．8g／L，MgSO4 0．2g／L，ZnSO4 0．03g／L，pH6．5。采用优化后的培养基条件，酶活力达到156．1U／L，比优化前初始发酵培养条件下的酶活提高了252％，当环氧化物水解酶催化时间为10h时，（s）-环氧氯丙烷的对映体过量值（e．e．）可达99．0％。产率为18．6％。

Screening, Identification, and Enzyme-Producing Conditions of Chiral Resolution of Epichlorohydrins Producing Strain

Five epoxide hydrolase-producing strains were isolated from soil samples, among them strain C375 was identified as Aspergillus niger ZJB-09103 by ITS sequence. The effects of carbon and nitrogen sources, metal ions, and pH etc. in the medium on the enzyme-production were observed, and obtained the optimized culture conditions as follow： starch 16 g/L, soybean meal 3 g/L, peptone 3 g/L, KH2PO4 0.4 g/L, K2HPO4 0.8 g/L, MgSO4 0.2 g/L, ZnSO4 0. 03 g/L, initial pH 6.5. The enzyme activity reached up to 156.1 U/L adopting the optimized culture conditions increased by 252% as compared with the one before the optimization. When the catalyzed time was 10 h with epoxide hydrolase the excess value of enantiomorph （e. e） of （S）-epiehlorohydrin could reach up to 99.0% at the production ratio of 18.6%.