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| 傣药植物内生真菌的抑菌活性评价及发酵条件探索 | |
| 引用本文： | 刘国强,赵立兴,赵国振,张金丽,熊智.傣药植物内生真菌的抑菌活性评价及发酵条件探索[J].微生物学杂志,2012,32(2):26-30. |
| 作者姓名： | 刘国强 赵立兴 赵国振 张金丽 熊智 |
| 作者单位： | 1. 西南林业大学西南山地森林保育与应用省部共建教育部重点实验室,云南昆明650224;云南大学省微生物研究所西南微生物多样性教育部重点实验室,云南昆明650091 2. 云南大学省微生物研究所西南微生物多样性教育部重点实验室,云南昆明,650091 3. 西南林业大学西南山地森林保育与应用省部共建教育部重点实验室,云南昆明,650224 |
| 摘 要： | 从云南傣药植物中分离到180株内生真菌,选用9种培养基进行发酵。利用茄腐镰刀菌、尖孢镰刀菌、立枯丝核菌、玉米小斑病、稻梨孢菌等5种植物病原菌作为指示菌株,结合TLC检测对其发酵粗提物进行活性评价和化学多样性分析,以期寻找到具有开发潜力的活性菌株。研究结果表明活性菌株为36株,其中有7株菌活性好且产物多样性丰富。 |
| 关 键 词： | 植物内生真菌 培养基 抑菌活性 筛选 |
Evaluation of Antibacterial Activity and Exploration of Fermentation Conditions of Dai Officinal Endophytic Fungi |
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| LIU Guo-qiang,ZHAO Li-xing,ZHAO Guo-zhen,ZHANG Jin-li,XIONG Zhi.Evaluation of Antibacterial Activity and Exploration of Fermentation Conditions of Dai Officinal Endophytic Fungi[J].Journal of Microbiology,2012,32(2):26-30. | |
| Authors: | LIU Guo-qiang ZHAO Li-xing ZHAO Guo-zhen ZHANG Jin-li XIONG Zhi |
| Institution: | 1.Key Lab.for Forest Res.Conserv.＆ Use in Upland Forest of SW China,Minist.of Educ.,SW Forest.Uni.,Kunming 650224; 2.Key Lab.of Microbial Diversity in SW China,Minist.of Educ.＆ Lab.for Conserv.＆ Util.of Bio-Res., Yunnan Inst.of Microbiol.,Yunnan Uni.,Kunming,650091) |
| Abstract: | 180 strains endophytic fungi were isolated from Yunnan Dai officinal,they were carried out fermentation using 9 kinds of medium.In order to find the active strains with development potential,fermentation extracts for their activity and chemical diversity were carried with five kinds of plant pathogen strains,Fusarium solani,F.oxysporum,Rhizoctonia solani,Bipolaris maydis,Pyricularia oryzae as indicator strain combined with TLC tests,The results showed that 36 strains were active,7 among them had fine activity of products diverse abundance. |
| Keywords: | endophytic fungi medium antibacterial activity screening |
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