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类球红细菌类胡萝卜素抗氧化活性研究
引用本文:安君,李祖明,张静,白志辉,杨卫东,王栋.类球红细菌类胡萝卜素抗氧化活性研究[J].微生物学杂志,2015(3):31-35.
作者姓名:安君  李祖明  张静  白志辉  杨卫东  王栋
作者单位:北京联合大学 应用文理学院,北京 100191;北京联合大学 应用文理学院,北京 100191;北京联合大学 应用文理学院,北京 100191;中国科学院 生态环境研究中心, 北京 100085;北京天维新探生物科技有限公司, 北京 100176;北京天维新探生物科技有限公司, 北京 100176
基金项目:北京市教育委员会科技计划面上项目(KM201311417007);北京市教育委员会科技成果转化和产业化项目(PXM2013_014209_07-000082)
摘    要:研究了研磨法、超声波法、酸溶辅助超声波法和菌体浓度对类球红细菌类胡萝卜素抗氧化活性的影响。结果表明,不同提取方法和固液比条件下,类球红细菌类胡萝卜素均具有清除DPPH自由基能力、抗脂质过氧化能力和还原能力。酸溶辅助超声波法提取的类胡萝卜素产率最高、抗氧化活性最好。类球红细菌类胡萝卜素具有一定的抗氧化活性,其抗氧化活性随菌体浓度的增加而增加。

关 键 词:类球红细菌  类胡萝卜素  酸溶辅助超声波法  抗氧化活性

Antioxidant Activity of Carotenoids from Rhodobacter sphaeroides
AN Jun,LI Zu-ming,ZHANG Jing,BAI Zhi-hui,YANG Wei-dong and WANG Dong.Antioxidant Activity of Carotenoids from Rhodobacter sphaeroides[J].Journal of Microbiology,2015(3):31-35.
Authors:AN Jun  LI Zu-ming  ZHANG Jing  BAI Zhi-hui  YANG Wei-dong and WANG Dong
Institution:Coll. of Appl. Arts & Sci. of Beijing Union Uni., Beijing 100191;Coll. of Appl. Arts & Sci. of Beijing Union Uni., Beijing 100191;Coll. of Appl. Arts & Sci. of Beijing Union Uni., Beijing 100191;The Res. Ctr. for Eco Environ. Sci., Chinese Acad. of Sci., Beijing 100085;Beijing Tianweixintan Biotechnol. Co. Ltd., Beijing 100176;Beijing Tianweixintan Biotechnol. Co. Ltd., Beijing 100176
Abstract:The effects of grinding, ultrasonic, ultrasonic assisted with acidolysis and thallus concentration on antioxidant activity of carotenoids from Rhodobacter sphaeroides were studied in this paper. The results showed that the carotenoids extracted with different methods and solid liquid proportion from R. sphaeroides 3757 possessed activities of scavenging DPPH free radicals, reducing power and resistance against lipid peroxidation. Carotenoids extracted by ultrasonic assisted with acidolysis from R. sphaeroides 3757 has the highest production rate and the best antioxidant activities. Caratenoids from R. sphaeroides possessed a certain antioxidant activities, and the antioxidant activities increased as the thallus concentration increased.
Keywords:Rhodobacter sphaeroides  carotenoids  ultrasonic assisted with acidolysis  antioxidant activity
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