杨树重要品种( 无性系) 的AFLP 指纹分析

品种的准确鉴定及其遗传相关性的了解对杨树育种和品种管理具有非常重要的意义。本试验采用AFLP对来自青杨组和黑杨组的21 个重要杨树品种( 无性系) 的鉴定与遗传相关性进行了研究。结果显示, 筛选的4对AFLP 引物总共产生了181 条多态性带,尤其是每对引物对每个品种都产生了独特的指纹图谱; 聚类分析和多维尺度分析将试验材料大体上分为五类, 结果不仅显示了组间不同品种的差异, 而且大体上区分了我国原生品种和外来品种。本研究表明, 所有品种都可被筛选的引物准确鉴定, 遗传相关性的推断结果与它们的系谱或分类基本一致。另外, 本研究还表明AFLP 技术完全可用于大规模地构建杨树树种DNA 指纹图谱、进行树种鉴定和遗传相关性的研究。

AFLP Fingerprinting of Elite Varieties ( clones) from the Genus Populus ( Populaceae)

Accurate identification of varieties ( clones) and understanding their genetic relationships are essential for the poplar breeding and variety management. In this study, 21 elite poplar varieties of Tacamahaca and Aigeiros were fingerprinted using AFLP markers. Four AFLP- primer pairs developed generated totally 181 AFLP polymorphic fragments, and in particular, each primer pair generated fingerprint profiles specific to each of the tested varieties. The genetic relationships among the varieties were evaluated by dendrograms and Multidimensional Scaling (MDS) , showing that the tested poplar can be classified into five groups. The results also indicated the clear- cup separation of varieties of different sections of poplar and the primary distinction between native and exotic poplar varieties. Present study indicated that tested poplar varieties could be identified by their fingerprint profiles and that genetic relationships deducted from the varieties study were consistent with their genealogy. In addition, our results demonstrated that AFLP could be used to construct DNA fingerprints of poplar clones at large- scale level and to determine genetic relationships of poplar varieties.