| 胰岛素B链C端及B29Lys氨基在胰岛素促生长功能中的作用 |
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| 引用本文： | 马建国,李默漪,郑积敏,崔大敷,冯佑民,景乃禾.胰岛素B链C端及B29Lys氨基在胰岛素促生长功能中的作用[J].生物化学与生物物理学报,1999(3). |
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| 作者姓名： | 马建国 李默漪 郑积敏 崔大敷 冯佑民 景乃禾 |
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| 作者单位： | 中国科学院上海生物化学研究所，中国科学院上海生物化学研究所分子生物学国家重点实验室 |
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| 摘 要： | 利用结晶紫测定细胞生长的方法，在９６孔板培养的小鼠乳腺癌细胞ＧＲ２Ｈ６中，建立了胰岛素促细胞生长活力的测定体系。该方法具有灵敏度高，重复性好，操作简便，周期短及便于大量样品分析测试的优点。利用该方法研究了胰岛素Ｂ链Ｃ端延长与缩短的类似物和胰岛素侧链氨基对胰岛素促生长活性的影响。结果显示，胰岛素Ｂ链Ｃ端在胰岛素的促生长活性中有重要作用；Ｂ链２９Ｌｙｓ氨基对促生长活性也有较大贡献。
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| 关 键 词： | 胰岛素 促生长活性 促生长测活体系 结晶紫 |
The Role of C Terminus of Insulin B Chain and the Amino Group of B29Lys Side Chain in the Growth Promoting Activities of Insulin |
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| MA Jian Guo,LI Mo Yi,ZHEN Ji Min,CUI Da Fu,FENG You Min and JING Nai He.The Role of C Terminus of Insulin B Chain and the Amino Group of B29Lys Side Chain in the Growth Promoting Activities of Insulin[J].Acta Biochimica et Biophysica Sinica,1999(3). |
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| Authors: | MA Jian Guo LI Mo Yi ZHEN Ji Min CUI Da Fu FENG You Min and JING Nai He |
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| Institution: | MA Jian Guo,LI Mo Yi,ZHEN Ji Min,CUI Da Fu,FENG You Min 1 and JING Nai He * |
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| Abstract: | By growing the mouse mammary tumor derived cell line GR2H6 in 96 well plates, we have developed an in vitro bioassay for the growth promoting activities of insulin. This bioassay system offers several advantages over currently used alternatives, such as higher sensitivity, better reproducibility and the processing of many samples simultaneously. Using this method, the mitogenic activities of insulin and its analogues were studied. The analogue with elongated C tenminus of insulin B chain (Ins NH 2) had a higher mitogenic activity than insulin (130% of insulin). The mitogenic activities of analogues with B29Lys amino group blocked was one third of those with B29Lys amino group free, indicating that the C terminal part of B chain and the amino group of B29Lys were important for the growth promoting activities of insulin. |
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| Keywords: | Insulin growth promoting activities bioassay crystal violet |
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