| Transiently Expressed Short Hairpin RNA Targeting 126 kDa Protein of Tobacco Mosaic Virus Interferes with Virus Infection |
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| 作者姓名： | Zhao MM An DR Zhao J Huang GH He ZH Chen JY |
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| 作者单位： | Ming-Min ZHAO~(1，2)，De-Rong AN~(1*)，Jian ZHAO~2，Guang-Hua HUANG~3，Zu-Hua HE~4，and Jiang-Ye CHEN~3
~1 College of Plant Protection，Northwest Science and Technology University of Agriculture and Forestry，Yangling 712100，China;
~2 College of Agriculture，Yangtze University，Jingzhou 434025，China;
~3 State Key Laboratory of Molecular Biology，Institute of Biochemistry and Cell Biology，Shanghai Institutes for Biological Sciences，Chinese Academy of Sciences，Shanghai 200031，China;
~4 State Key Laboratory of Plant Molecular Genetics，Institute of Plant Physiology and Ecology，Shanghai Institutes for Biological Sciences，Chinese Academy of Sciences，Shanghai 200032，China |
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| 摘 要： | RNA-interference (RNAi) silences gene expression by'guiding mRNA degradation in asequence-specific fashion.Small interfering RNA (siRNA),an intermediate of the RNAi pathway,has beenshown to be very effective in inhibiting virus infection in mammalian cells and cultured plant cells.Here,wereport that Agrobacterium tumefaciens-mediated transient expression of short hairpin RNA (shRNA) couldinhibit tobacco mosaic virus (TMV) RNA accumulation by targeting the gene encoding the replication-asso-ciated 126 kDa protein in intact plant tissue.Our results indicate that transiently expressed shRNA efficientlyinterfered with TMV infection.The interference observed is sequence-specific,and time-and site-dependent.Transiently expressed shRNA corresponding to the TMV 126 kDa protein gene did not inhibit cucumbermosaic virus (CMV),an unrelated tobamovirus.In order to interfere with TMV accumulation in tobaccoleaves,it is essential for the shRNA constructs to be infiltrated into the same leaves as TMV inoculation.Ourresults support the view that RNAi opens the door for novel therapeutic procedures against virus diseases.We propose that a combination of the RNAi technique and Agrobacterium-mediated transient expressioncould be employed as a potent antiviral treatment in plants.
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| 关 键 词： | 126 kDa 烟草病毒 病毒感染 基因表达 |
| 收稿时间： | 2005-08-04 |
| 修稿时间： | 2005-10-21 |
Transiently expressed short hairpin RNA targeting 126 kDa protein of tobacco mosaic virus interferes with virus infection |
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| Zhao MM,An DR,Zhao J,Huang GH,He ZH,Chen JY.Transiently Expressed Short Hairpin RNA Targeting 126 kDa Protein of Tobacco Mosaic Virus Interferes with Virus Infection[J].Acta Biochimica et Biophysica Sinica,2006,38(1):22-28. |
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| Authors: | Zhao Ming-Min An De-Rong Zhao Jian Huang Guang-Hua He Zu-Hua Chen Jiang-Ye |
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| Institution: | College of Plant Protection, Northwest Science and Technology University of Agriculture and Forestry, Yangling 712100, China. |
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| Abstract: | RNA-interference (RNAi) silences gene expression by'guiding mRNA degradation in a sequence-specific fashion.Small interfering RNA (siRNA),an intermediate of the RNAi pathway,has been shown to be very effective in inhibiting virus infection in mammalian cells and cultured plant cells.Here,we report that Agrobacterium tumefaciens-mediated transient expression of short hairpin RNA (shRNA) could inhibit tobacco mosaic virus (TMV) RNA accumulation by targeting the gene encoding the replication-asso- ciated 126 kDa protein in intact plant tissue.Our results indicate that transiently expressed shRNA efficiently interfered with TMV infection.The interference observed is sequence-specific,and time-and site-dependent. Transiently expressed shRNA corresponding to the TMV 126 kDa protein gene did not inhibit cucumber mosaic virus (CMV),an unrelated tobamovirus.In order to interfere with TMV accumulation in tobacco leaves,it is essential for the shRNA constructs to be infiltrated into the same leaves as TMV inoculation.Our results support the view that RNAi opens the door for novel therapeutic procedures against virus diseases. We propose that a combination of the RNAi technique and Agrobacterium-mediated transient expression could be employed as a potent antiviral treatment in plants. |
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| Keywords: | tobacco mosaic virus 126 kDa protein RNA interference short hairpin RNA |
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