| 天花粉蛋白诱发白血病细胞K562凋亡的研究 |
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| 引用本文： | 孔梅,柯一保,周美云,柯欣永,吕飚,聂慧玲.天花粉蛋白诱发白血病细胞K562凋亡的研究[J].分子细胞生物学报,1998(3). |
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| 作者姓名： | 孔梅 柯一保 周美云 柯欣永 吕飚 聂慧玲 |
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| 作者单位： | 中国科学院上海细胞生物学研究所，中国科学院上海细胞生物学研究所，中国科学院上海细胞生物学研究所，中国科学院上海细胞生物学研究所，中国科学院上海细胞生物学研究所，中国科学院上海细胞生物学研究所 上海 200031，上海 200031，上海 200031，上海 200031，上海 200031，上海 200031 |
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| 摘 要： | 天花粉蛋白(Trichosanthin,TCS),是一种从栝楼块根内提取的核糖体失活蛋白,具有流产、抗肿瘤和抗HIV等多种生物活性。本文利用FACS检测到天花粉蛋白可使K562白血病细胞产生明显的凋亡小峰、DNA区带电泳成典型的“梯状”条带,电镜检测可观察到明显的细胞凋亡形态。这些结果表明天花粉蛋白可以诱发K562白血病细胞产生凋亡。
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| 关 键 词： | 天花粉蛋白 白血病细胞K562 细胞凋亡 |
STUDY ON TRICHOSANTHIN INDUCED APOPTOSIS OF LEUKEMIA K562 CELLS |
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| KONG Mei KE Yi Bao ZHOU Mei Yun KE Xin Yong LU Biao NIE Hui Ling.STUDY ON TRICHOSANTHIN INDUCED APOPTOSIS OF LEUKEMIA K562 CELLS[J].Journal of Molecular Cell Biology,1998(3). |
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| Authors: | KONG Mei KE Yi Bao ZHOU Mei Yun KE Xin Yong LU Biao NIE Hui Ling |
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| Abstract: | Trichosanthin (TCS), an eukaryotic ribo-some-inactivating protein isolated from the root tuber of Trichosanthes plant, has various biological activities including abortion induction, anti-tumor, and anti-HIV. In this study, cultured human leukemia K562 cells treated with trichosan-thin were examined. Analysis of the cells by single laser flow cytometry showed the sub-G1 peak.
DNA extracted from these cells formed a characteristic "ladder" on agarose gel electrophoresis. Under electromicroscope, typical morphological changes of apoptosis were also observed. From all of these findings, we concluded that tri-chosanthin was able to induce apoptosis in K562 cells. |
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| Keywords: | Trichosanthin Leukemia K562 Cells Cell Apoptosis |
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