Type-I collagen production by human odontoblast-like cells in expiants cultured on cyanoacrylate films |
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| Authors: | Prof H Magloire A Callé D J Hartmann A Joffre B Serre J A Grimaud F Schué |
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| Institution: | (1) Laboratoire d'Histophysiologie et de Pathologie des Tissus Dentaires U.A., Lyon and C.M.E.A.B.G.; Faculté des Sciences, CNRS 819, Faculté d'Odontologie Villeurbanne, France;(2) Centre de Radioanalyse, U.A., CNRS 819, Institut Pasteur, Lyon, France;(3) Laboratoire de Chimie Macromoléculaire, Université des Sciences et des Techniques, Montpellier, France;(4) Laboratoire de Pathologie Cellulaire du Foie, U.A., CNRS 819, Institut Pasteur, Lyon, France;(5) Gaculté d'Odontologie, rue G. Paradin, 69372 Lyon Cedex 2, France |
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| Abstract: | Summary Odontoblast-like cells derived from human tooth pulps were maintained in expiant culture and grown either on glass coverslips only (used as control) or on glass coverslips coated with cyanoacrylate films. Ultrastructural and cyto-morphometric evidence showed that cells exposed to cyanoacrylate, in contrast to controls, display a significant decrease of rough endoplasmic reticulum and mitochondria. In addition, immunofluorescent staining and radioimmunoassays for type-I collagen suggested disturbances in production for the exposed cells. The use of anti-fibronectin antibodies with electron-microscopic immunoperoxidase-labelling demonstrated that the adherence of cells to cyanoacrylate can involve both adhesion plaques and fibronectin. These results therefore suggest that there were no apparent differences in the adhesion interaction of cells between glass and cyanoacrylate substrates. |
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| Keywords: | Collagen Odontoblast Cyanoacrylate Fibronectin Human |
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