Splenic primary sensory afferents in the guinea pig demonstrated with anterogradely transported wheat-germ agglutinin conjugated to horseradish peroxidase |
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Authors: | Lars-Gösta Elfvin Håkan Aldskogius Jan Johansson |
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Institution: | (1) Department of Anatomy, Karolinska Institutet, P.O. Box 60400, S-104 01 Stockholm, Sweden |
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Abstract: | Summary The distribution of primary visceral afferents to the spleen of the guinea pig was studied after injections of wheat-germ agglutinin conjugated to horseradish peroxidase (HRP) into the left dorsal root ganglia at levels T7–T12. After anterograde transport of the tracer, labeled fibers were found in the nerves around the splenic artery in the hilus region and in the splenic parenchyma. The majority of labeled fibers in the spleen were detected in the white pulp. HRP-positive fibers were also observed in the red pulp and in the trabeculae. The distribution of the HRP-labeled fibers was in part similar to those of substance P-immunoreactive and calcitonin gene-related peptide-immunoreactive nerve structures. The results show that the anterograde tracing technique can be used successfully to investigate splenic primary afferent innervation. |
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Keywords: | Spleen Splenic nerve Primary sensory neurons Lectin Horseradish peroxidase conjugate Anterograde axonal transport Guinea pig |
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