Parametric Studies on Batch Degradation of a Plasticizer Di-n-Butylphthalate by Immobilized Bacillus sp |
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Authors: | Neelakanteshwar K Patil T B Karegoudar |
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Institution: | (1) Department of Biochemistry, Gulbarga University, Gulbarga, 585 106, Karnataka, India |
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Abstract: | Summary Di-n-butylphthalate (DBP) is one of the phthalate esters (PAEs) used in the manufacture of plasticizers, insect repellents and
synthetic fibres and contributes to environmental pollution. We report a novel bacterium belonging to the genus, Bacillus (NCIM 5220), which has the ability to utilize DBP as the sole source of carbon and energy. This bacterium was immobilized
in alginate. The degradation of DBP by immobilized cells was compared with free cells. The effects on the degradation of DBP
of different factors like gel (alginate) concentration, gel bead size, temperature, and pH were investigated. Oxygen uptake
in the presence of DBP by free and immobilized cells was also studied. The results showed that the degradation of DBP by immobilized
cells was more efficient than by free cells. Further, the effect of various factors tested on the degradation of DBP by alginate-immobilized
cells showed that the degradation of DBP was remarkably affected by alginate concentration between 2 and 5% and drastically
decreased between bead size 2 and 5 mm. A change of 10 °C of reaction temperature from 30 to 40 °C did not alter the degradation
of DBP, and maximum degradation was appeared to be favoured over a broad pH range of 6.5–7.5 for immobilized cells as compared
to free cells, which showed an optimum temperature of about 35 °C and pH of 7.0. The immobilized cells showed higher oxidation
of DBP than free cells. Thus more efficient degradation of DBP could be achieved by immobilizing Bacillus sp. in alginate beads. |
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Keywords: | Alginate Bacillus sp degradation di-n-butyl phthalate immobilization parametric study phthalate esters |
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