| 花生RGA 片段的克隆及初步分析 |
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| 引用本文： | 黄湘文,张冲 石新国 陈华 郑奕雄.花生RGA 片段的克隆及初步分析[J].现代生物医学进展,2011,11(9):1631-1633. |
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| 作者姓名： | 黄湘文 张冲 石新国 陈华 郑奕雄 |
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| 作者单位： | 1. 仲恺农业工程学院,广东,广州,510225;福建农林大学油料作物研究所,福建,福州,350002
2. 福建农林大学油料作物研究所,福建,福州,350002 |
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| 摘 要： | 目的:通过同源克隆获得了花生闽花6号的RGA片段,为其抗性的研究及抗性育种提供了参考资料。方法:试验分为两组:其一通过利用抗性基因的NBS保守区所设计的简并引物对花生品种闽花六号进行了RGA片段扩增,其二结合已登录的花生RGA片段序列经过多元比对后设计简并引物进行RGA片段的扩增及序列分析;分析比较两组克隆方法的效果。结果:测序分析表明:前者20条随机测序序列中没有一条与已知RGA片段序列相似;后者20条随机测序序列中有18条为RGA片段序列,其登录号为GenBankEU639668-EU639685。结论:前一种方法克隆扩增RGA基因片段的效率很低,而后一种方法克隆扩增效果更好,这为闽花6号花生的遗传改良提供了理论基础。
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| 关 键 词： | 花生 RGA 同源克隆 |
Cloning and Primary Analysis for RGA Fragments in Peanut |
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| HUANG Xiang-wen,ZHANG Chong,SHI Xin-guo,CHEN Hu,ZHENG Yi-xiong.Cloning and Primary Analysis for RGA Fragments in Peanut[J].Progress in Modern Biomedicine,2011,11(9):1631-1633. |
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| Authors: | HUANG Xiang-wen ZHANG Chong SHI Xin-guo CHEN Hu ZHENG Yi-xiong |
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| Institution: | HUANG Xiang-wen1,2,ZHANG Chong2,SHI Xin-guo2,CHEN Hua2,ZHENG Yi-xiong1,2 (1 Zhongkai University of Agriculture and Engineering,Guangzhou,510225,2 College of oil Crop Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China) |
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| Abstract: | Objective: RGA fragments were cloned to investigate resistance and provide theoretical guidance for breeding in peanut. Methods: In this study, degenerate PCR primers firstly designed to bind to DNA regions encoding conserved motifs within NBS (nucleotide binding site) were used to amplify NBS-encoding regions from A. hypogaea L. Minhua Number 6. At the same time a pair of degenerate primers was designed according to the result of multiple sequence alignment from known RGA sequences in peanut. RGA fragments... |
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| Keywords: | Peanut RGA homology cloning |
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