中国医学科学院北京协和医学院放射医学研究所天津市分子核医学重点实验室

血液肿瘤即造血系统的恶性肿瘤,是一种严重危害公共健康的疾病。目前,血液肿瘤诊断治疗的最理想方法就是分子特异性诊断和靶向治疗,但该方法面临的最大困难就是分子靶点的选择。噬菌体展示技术是近十年发展起来的一种新的生物学技术,具有高通量筛选、模拟天然表位、易于纯化、将蛋白功能与编码基因相统一等优点,广泛应用于功能性蛋白质和多肽的筛选、蛋白质间的识别与相互作用、抗原表位的鉴定、基因工程抗体的筛选等多个分子生物学领域,非常适于理想靶点的选择。目前,噬菌体文库技术在血液肿瘤诊治中的应用主要集中在噬菌体抗体文库和噬菌体随机肽库上。本文就噬菌体展示技术在血液肿瘤诊断治疗中的研究成果做一总结分析,并对该技术在这一领域的应用前景进行展望。

The Application of Phage Display Technology in the Diagnosis and Therapy of Hematological Malignancies

Hematopoietic malignancies are serious hazard to public health.Presently,the most ideal method in diagnosing and treating hematopoietic malignancies is specific molecular diagnosis and targeted therapy.However,the most stubborn difficulty of this method lies in the screening of molecular target.Phage display technology,a powerful approach developed in recent decade,has many advantages,including high throughput screening,mimicking natural epitopes,easy purification,integrating protein function with its coding gene,etc.Phage display technology is widely used in the area of molecular biology,such as screening of functional peptides and proteins,the study of protein interactions,identification of antigens,screening of genetically engineered antibodies,etc,which is very fit for the screening of ideal targets.At present,the application of this technology in hematopoietic malignancies diagnosis and therapy is focus on phage antibody library and phage random peptide library.This article will introduce the research findings of phage display technology in hematopoietic malignancies diagnosis and therapy,and look forward to the prospect of this technology in this area.