| 大鼠膝骨关节炎滑膜细胞原代培养 |
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| 引用本文： | 袁琴,阚卫兵,宋朋飞,张隆,王拥军.大鼠膝骨关节炎滑膜细胞原代培养[J].现代生物医学进展,2012,12(2):253-255. |
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| 作者姓名： | 袁琴 阚卫兵 宋朋飞 张隆 王拥军 |
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| 作者单位： | 1. 上海中医药大学附属普陀医院伤骨科 上海20006
2. 上海中医药大学脊柱病研究所 上海200032 |
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| 基金项目： | 上海市教委课题资助(08cz041);上海市教委高校创新团队课题资助[沪教委科(2009)6号];上海普陀区科委卫生系统自主创新基金资助 |
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| 摘 要： | 目的:建立原代培养膝骨关节炎大鼠滑膜细胞的方法。方法:取膝骨关节炎大鼠膝关节滑膜组织,用酶消化法消化、分离细胞,用DMEM培养液进行培养并传代,观察滑膜细胞生长状况、并用细胞形态学及细胞免疫化学鉴定。结果:用酶消化法培养膝骨关节炎滑膜细胞,方法简单、成功率高、细胞形态典型。结论:单一的胶原酶消化法是一种有效的滑膜细胞原代培养的方法,可以满足一般实验需求。
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| 关 键 词： | 滑膜细胞 体外培养 膝骨关节炎 |
Primary Culture of Rats Synoviocytes with Knee of Osteoarthritis |
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| YUAN Qin,KAN Wei-bing,ZHANG Long,SONG Peng-fe,WANG Yong-jun.Primary Culture of Rats Synoviocytes with Knee of Osteoarthritis[J].Progress in Modern Biomedicine,2012,12(2):253-255. |
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| Authors: | YUAN Qin KAN Wei-bing ZHANG Long SONG Peng-fe WANG Yong-jun |
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| Institution: | 1 Department of orthopedic injuries,Putuo Hospital,Shanghai University of Traditional Chinese Medicine,200062,Shangshai,China; 2 Institute of Spine Disease,Shanghai University of Traditional Chinese Medicine,200032,Shangshai,China) |
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| Abstract: | Objective: To develop a digestive method to obtain rats synoviocytes with knee of Osteoarthritis.Methods: Rats of OA joint synovial layers were cut into small pieces and digested with collagenase.Cell suspension was cultured in DMEM containing 20% fatal bovine serum.Cells were identified by morphology and immunochemistry analysis.Results: The appearance of synoviocytes is typi-cal and the method is simple.Conclusions: Single collagenase digestion is effective for isolation of synoviocytes,and can satisfy the gen-eral test requirements. |
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| Keywords: | Synoviocytes Culture in vitro Knee Osteoarthritis |
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