混合甲醛固定液固定大肠癌淋巴结标本的最佳免疫组化效果研究

目的:探讨混合甲醛固定液固定大肠癌淋巴结标本的最佳免疫组化效果。方法:采用不同pH值(6.0、7.0、8.0)的混合甲醛固定液对39枚大肠癌淋巴结标本进行不同时间(6 h、6 h-12 h、1 d-7 d)的固定处理。以细胞角蛋白20(CK20)为目标抗原,运用OIympusdp 70图像采集分析仪抽选出混合甲醛固定液最佳免疫组化染色的pH值及固定时间。结果:经pH值为7.0混合甲醛固定液处理后,阳性率为92.31%,高于经pH值为6.0、8.0的混合甲醛固定液处理后的76.92%、74.36%,且经pH值为7.0、8.0处理后的阳性率比较有统计差异(P0.05)。混合甲醛固定液的固定时间在6 h-12 h时的阳性率为94.87%,高于固定时间为6 h、1 d-7 d处理的30.77%、76.92%(P0.05)。结论:对于大肠癌淋巴结标本,以CK20为目标抗原,选择pH值为7.0的混合甲醛固定液固定6 h-12 h能够得到质量较佳的免疫组化染色效果。

Best Immunohistochemical Effect of Mixed Formaldehyde Fixative for Colorectal Cancer Lymph Node Specimens

ABSTRACT Objective: To investigate the best immunohistochemical effect of mixed formaldehyde fixative for colorectal cancer lymph node specimens. Methods: A total of 39 cases of colorectal cancer lymph node specimens were fixed treatment at different time (<6 h, 6 h-12 h,1 d-7 d) by different pH values (6.0,7.0,8.0) of mixed formaldehyde fixative. The cytokeratin 20 (CK20) was used as the target antigen. OIympusdp 70 image acquisition analyzer was used to extract the best pH value and fixation time of mixed formaldehyde fixative. Results: After mixed formaldehyde fixative treated by pH values 7.0, the positive rate was 92.31%, which was higher than 76.92% and 74.36% of mixed formaldehyde fixative treated by pH values 6.0 and 8.0 respectively. And there were statistical differences between the positive rate after treated by pH values 7.0 and 8.0(P<0.05). The positive rate of mixed formaldehyde fixative at 6 h-12 h was 94.87%, higher than 30.77% and 76.92% at the fixed time less than 6 h and 1 d-7 d. Conclusion: For colorectal cancer lymph node specimens, CK20 is used as the target antigen, a better quality of immunohistochemical stain effect could be obtained by fixing for 6 h-12 h with mixed formaldehyde fixative of pH values 7.0.