葛根素联合小檗碱干预非酒精性脂肪性肝炎两种动物模型的病理学研究

目的:从病理学的角度探讨葛根素及小檗碱对非酒精性脂肪性肝炎(NASH)的干预作用。方法:制备高脂饮食诱导的SD大鼠NASH模型及蛋氨酸-胆碱缺乏饮食诱导的C57BL/6小鼠NASH模型,各给药组造模并同时灌胃给药,大鼠持续8周后取材,小鼠持续2周后取材。肝组织制成石蜡切片进行HE染色,制成冰冻切片进行油红O染色,经光镜观察摄片后,采用NAFLD活动度积分对各组进行NASH评估。结果:HE及油红O检测提示葛根素联合小檗碱可减轻两种NASH动物模型肝脏的脂肪变性、抑制肝细胞空泡化的发生,减少肝小叶内炎性病灶的数量。给予葛根素联合小檗碱的SD大鼠NAS总分为2.60±0.52(3),给予葛根素联合小檗碱的C57BL/6小鼠NAS总分为2.80±0.79(3),均已排除NASH。结论:葛根素联合小檗碱可显著抑制NASH相关病理变化的发生发展,对NASH具有一定的干预作用。

Pathological Study of Puerarin Combined with Berberine on Two Animal Models of Non-alcoholic Steatohepatitis

ABSTRACT Objective: To investigate the effect of puerarin and berberine on non-alcoholic steatohepatitis (NASH) from the pathological point of view. Methods: The NASH model of SD rats induced by high fat diet, the NASH model of C57BL/6 mice induced by methionine-choline deficiency diet were prepared. Each treatment group was given intragastric administration while being modeled. The rats were dissected after 8 weeks and the mice were dissected after 2 weeks. Paraffin sections of liver tissue were stained with HE and frozen sections were stained with oil red O. After light microscopy, NAFLD activity score was used to evaluate NASH in each group. Results: HE and oil red O assay showed that puerarin combined with berberine could alleviate steatosis, inhibit vacuolation of hepatocytes and reduce the number of inflammatory lesions in hepatic lobules in two NASH animal models. The total NAS of SD rats treated with puerarin and berberine was 2.60 +0.52 (< 3), and the total NAS of C57BL/6 mice treated with puerarin and berberine was 2.80 +0.79 (< 3), which excluded NASH. Conclusion:Puerarin and berberine can significantly inhibit the occurrence and development of pathological changes related to NASH. They have a certain intervention effect on NASH.