| 海鲈核糖体蛋白L8cDNA的克隆与进化分析 |
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| 引用本文： | 丛潇,邵占涛,袁金铎,王友政,杨桂文,安利国.海鲈核糖体蛋白L8cDNA的克隆与进化分析[J].现代生物医学进展,2008,8(5):858-861. |
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| 作者姓名： | 丛潇 邵占涛 袁金铎 王友政 杨桂文 安利国 |
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| 作者单位： | 山东师范大学生命科学院,动物抗性重点试验室,山东,济南,250014 |
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| 基金项目： | 国家自然科学基金
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教育部高校博士学专项科研基金 |
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| 摘 要： | 本文构建了海鲈(Lateolabrax japonicus)头肾全长eDNA文库.PCR方法扩增得到海鲈的核糖体蛋白L8基因,全长848bp,编码257个氨基酸,含有L2及L2-C两个保守区.进化分析结果表明,以L8为参照的进化鉴定结果同经典的分子生物学标准18s鉴定结果十分相似,因此核糖体蛋白L8基因L8可以作为鉴定物种进化程度的新标准.
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| 关 键 词： | 核糖体蛋白L8 鲈鱼 cDNA文库 进化标准 Ribosomal protein L8 Lateolabrax japonicus cDNA library Phylogenetic criterion 核糖体 蛋白 克隆 进化分析 Protein Phylogenetic Analysis Cloning japonicus high similarity supports used phylogenetic analysis criterion phylogenetic relationship rDNA consistent role alignment quite |
| 文章编号： | 1673-6273(2008)05-0858-04 |
| 修稿时间： | 2008年2月1日 |
Cloning and Phylogenetic Analysis of the Ribosomal Protein L8 cDNA of the Weever(Lateolabrax japonicus) |
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| CONG Xiao,SHAO Zhan-tao,YUAN Jin-duo,WANG You-zheng,YANG Gui-wen,An Li-guo.Cloning and Phylogenetic Analysis of the Ribosomal Protein L8 cDNA of the Weever(Lateolabrax japonicus)[J].Progress in Modern Biomedicine,2008,8(5):858-861. |
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| Authors: | CONG Xiao SHAO Zhan-tao YUAN Jin-duo WANG You-zheng YANG Gui-wen An Li-guo |
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| Abstract: | The complete nucleotide sequence of a cDNA clone encoding weever ribosomal protein L8, WeevL8, is described here. The WeevL8 cDNA has 848 nucleotides in full length and encodes a 257 amino-acid protein with a calculated molecular mass of 28.02 kDa. Two conserved domains have been identified in WEEVL8. It was concluded fromsequence alignment that WeevL8 gene was quite conservative, consistent with its role as a house-keeping gene. A phylogenetic analysis made by L8 protein showed the similar phylogenetic relationship to that with 18s rDNA. The high similarity supports the notion that ribosomal protein L8 can also be used as phylogenetic criterion. |
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| Keywords: | Ribosomal protein L8 Lateolabrax japonicus cDNA library Phylogenetic criterion |
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