近红外荧光染料IR-783在膀胱癌中的成像研究

目的:观察研究近红外荧光染料IR-783在膀胱癌中的特异性成像。方法:通过染料与膀胱癌细胞及正常细胞共孵育,观察近红外荧光染料IR-783是否能够实现膀胱癌细胞的选择性成像。利用细胞器示踪剂观察近红外荧光染料在膀胱癌细胞内的共定位;使用IR-783检测循环血液中及尿液中的膀胱癌细胞。结果:近红外荧光染料IR-783可被膀胱癌细胞选择性摄取。IR-783可选择性聚集在膜性细胞器如线粒体和溶酶体内,这种选择性聚集作用使IR-783可以保持较长的染色效果。近红外染料可以检测到血液或尿液极少量的膀胱癌细胞。结论:近红外荧光染料IR-783能够被膀胱癌细胞特异性吸收,可用于血液和尿液中膀胱肿瘤细胞的特异性诊断,具有重要的临床应用前景。

IR-783 Dye for Near-Infrared Fluorescence Imaging in Bladder Cancer

ABSTRACT Objective: To observe the effect of near infrared fluorescent dye IR-783-mediated specific tumor imaging in bladder cancer. Methods: The ability of IR-783 to accumulate in bladder cancer cell was determined through bladder cancer cells and normal cells were stained. Subcellular co-localization study of IR-783 in bladder cancercells by Organelles tracer. IR-783-mediated imaging of bladder cancer in human blood and urine. Results: Bladder cancer cells selectively uptake near infrared fluorescent dye IR-783. IR-783 can selectively aggregated in mitochondria and lysosomes, which contribute to maintain longer staining of IR-783. Near infrared fluorescence could detect few bladder cancer cells in human blood and urine samples. Conclusion: Near infrared fluorescence IR-783 could be specifically taken up by bladder cancer, and can be used for specific diagnosis of bladder cancer cells in human blood and urine. It has an important clinical application prospect.