上调OPN和TIP30表达对口腔鳞癌cal-27细胞的影响

目的:探讨上调骨桥蛋白(OPN)和TIP30表达对口腔鳞状细胞癌cal-27细胞增殖和迁移能力的影响。方法:采用过表达OPN载体病毒和过表达TIP30质粒分别感染或转染cal-27细胞株后,通过实时荧光定量PCR法和蛋白印迹western blot法检测OPN和TIP30的表达,CCK-8法检测细胞增殖能力,细胞划痕实验检测细胞的迁移能力。结果:过表达OPN载体病毒感染的cal-27-sh OPN细胞株OPN m RNA和蛋白的表达均明显高于cal-27-sh N细胞株(P0.001,P=0.002),且细胞的增殖能力和迁移能力强(P0.001,P=0.002)。过表达TIP30质粒转染的cal-27-p TIP30细胞株TIP30 m RNA和蛋白的表达均明显高于cal-27-pc3.0细胞株(P=0.001,P=0.003),且细胞的增殖能力和迁移能力弱(P0.001,P=0.005)。结论:OPN能促进cal-27细胞的增殖和迁移,TIP30抑制cal-27细胞的增殖和迁移。

The Influence of up-regulated Expressions of TIP30 and OPN on the Oral Squamous Cell Carcinoma Cal-27 Cells

ABSTRACT Objective: To study the influence of up-regulated expressions of OPN (osteopontin) or TIP30 on the oral squamous cell carcinoma cal-27 cells. Methods: The TIP30 and OPN were overexpressed in the cal-27 cell lines respectively by infection or transfection technology. Real-time fluorescent quantitative PCR (RT-PCR) and western blot were used to detect the TIP30 and OPN mRNA and protein expressions. The proliferation ability was tested by CCK-8 tests, while the migration ability was tested by cell scratch experiment tests. Results: The expression of OPN mRNA and protein in cal-27-shOPN cell line was higher than those in the cal-27-shN cell lines(P<0.001, P=0.002). The proliferation and migration ability of cal-27-shOPN cell line were better than those of cal-27-shN cell line (P<0.001, P=0.002). The expression of TIP30 mRNA and protein in cal-27-pTIP30 cell line were higher than in the cal-27-pc3.0 cell line (P=0.001, P=0.003). The proliferation and migration ability of cal-27-pTIP30 cell line were worse than those of the cal-27-pc3.0 cell line (P<0.001, P=0.005). Conclusion: OPN could improve the ability of proliferation and migration of cal-27 cell line, while TIP30 could reduce the ability of proliferation and migration of cal-27 cell line.