血清白介素8基因多态性与食管鳞癌根治术后的相关性分析

摘要 目的：探讨与分析血清白细胞介素8（IL-8）基因多态性与食管鳞癌（ESCC）根治术后的相关性。方法：2017年8月到2020年6月选择在本院诊治的食管鳞癌患者98例作为研究对象，检测血清IL-8表达水平。所有患者都给予根治手术治疗，随访患者的预后并进行相关性分析。结果：所有患者术后随访到2021年7月，平均随访时间为25.69±2.58个月，死亡28例，死亡率为28.6 %（死亡组）。两组血清IL-8表达水平表达具有差异（P<0.05）。所有患者的基因型频率均符合Hardy-Weinberg这一平衡法则，表明本文所选取的样本均具有群体代表性。IL-8基因启动子rs4073A/T的AA基因型较死亡组高，TT基因型较死亡组低，两组A、T等位基因频率分布对比有差异（P<0.05）。直线相关性分析显示：IL-8基因启动子rs4073A/T的AA基因型、A等位基因、血清IL-8表达水平与预后死亡率存在相关性（P<0.05）。多因素logistic回归分析显示：IL-8基因启动子rs4073A/T的AA基因型、A等位基因、血清IL-8表达水平为导致患者随访死亡的主要因素（OR=2.051，3.094，P<0.05）。结论：食管鳞癌根治术后患者依然存在一定的死亡率，患者死亡与血清IL-8基因多态性存在相关性，同时多伴随有IL-8的高表达。IL-8基因启动子rs4073A/T的AA基因型、A等位基因、血清IL-8表达水平为导致患者死亡的主要因素。

Correlation Analysis between Serum Interleukin-8 Gene Polymorphism and Radical Resection of Esophageal Squamous Cell Carcinoma

ABSTRACT Objective: To explore and analyze the correlation between serum IL-8 gene polymorphism and ESCC after radical resection. Methods: From August 2017 to June 2020, 98 cases of patients with esophageal squamous cell carcinoma diagnosed and treated in our hospital were selected as the research object, and the serum IL-8 expression level were detected. All patients were treated with radical surgery, and the prognosis of the patients were followed up and given correlation analysis. Results: All patients were followed up to July 2021. The average follow-up time were 25.69±2.58 months. There were 28 deaths and the mortality rate were 28.6 % (death group). The expression level of serum IL-8 in the death group were higher than that in the survival group(P<0.05). The genotype frequencies of all patients were conformed to the Hardy-Weinberg equilibrium rule, which confirmed that the samples were representative of the population. The AA genotype of the IL-8 gene promoter rs4073A/T were higher than that of the death group(P<0.05), and the TT genotype were lower than that of the death group(P<0.05). There were also statistical differences in the frequency distribution of A and T alleles compared between the two groups(P<0.05). Linear correlation analysis showed that the AA genotype and A allele of IL-8 gene promoter rs4073A/T, expression level of serum IL-8 were correlated with prognosis and mortality(P<0.05). Multivariate logistic regression analysis showed that the AA genotype and A allele of IL-8 gene promoter rs4073A/T, expression level of serum IL-8 were the main factors leaded to followed-up death (OR=2.051, 3.094, P<0.05). Conclusion: Patients with esophageal squamous cell carcinoma still have certain mortality rate after radical resection. There arecorrelation between the death of patients and the polymorphism of serum IL-8 gene. It are also often accompanied by high expression of IL-8. The AA genotype and A allele of IL-8 gene promoter rs4073A/T, expression level of serum IL-8 are The main factor lead to the death of patients.