| HPLC法测定花生根中白藜芦醇的含量 |
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| 引用本文： | 向海艳,周春山,雷启福,肖建波,陈龙胜.HPLC法测定花生根中白藜芦醇的含量[J].天然产物研究与开发,2005,17(2):179-181. |
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| 作者姓名： | 向海艳 周春山 雷启福 肖建波 陈龙胜 |
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| 作者单位： | 中南大学化学化工学院,长沙,410083 |
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| 基金项目： | ThisprojectwassupportedbytheKeyProgramofSci enceandTechnologyOfficeofHunanProvince(0 0KNY10 10 ) |
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| 摘 要： | 本文采用反相高效液相色谱法测定了花生根中白藜芦醇的含量.选用ODS-C18色谱柱(200
mm×4.6mm,5μm),以甲醇-水(4555)为流动相,流速为0.5 mL/min,紫外检测波长为303nm.结果表明,白藜芦醇峰面积与其浓度之间呈现良好的线性关系,线性回归方程为Y=15879.28X+12350.2,相关系数为0.9996.方法简单,快速,精密度好,回收率为98.8%,相对标准偏差为1.79%.
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| 关 键 词： | 高效液相色谱法 花生 根 白藜芦醇 含量测定 |
Determination of Resveratrol in Peanut Root by HPLC |
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| XIANG Hai-yan,ZHOU Chun-shan,LEI Qi-fu,XIAO Jian-bo,CHEN Long-sheng.Determination of Resveratrol in Peanut Root by HPLC[J].Natural Product Research and Development,2005,17(2):179-181. |
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| Authors: | XIANG Hai-yan ZHOU Chun-shan LEI Qi-fu XIAO Jian-bo CHEN Long-sheng |
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| Institution: | XIANG Hai-yan,ZHOU Chun-shan*,LEI Qi-fu,XIAO Jian-bo,CHEN Long-sheng |
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| Abstract: | A resversed-phase high perrormance liquid chromatography(HPLC)method was developed to determine resveratrol in peanut root. The separation was performed on an ODS column(200 × 4.6 mm,5 μm), with the mobile phase of CH3OH-H2O(45:55,v/v).The flow rate was 0.5 mL/min and the detection wavelength was 303 nm. The result showed that there was a good linear relationship between the area and the concentration of resveratrol. The corresponding regression equation is Y =15879.28X + 12350.2,R= 0.9996.The proposed method is simple and rapid with good precion. The average recovery is 98.8% and RSD is 1.79%. |
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| Keywords: | HPLC resveratrol peanut roots |
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