不同品种淫羊藿黄酮类有效成分的主成分分析及对肾阳虚大鼠保护作用的研究

采用HPLC测定不同品种淫羊藿黄酮类即淫羊藿苷、朝藿定A、朝藿定B、朝藿定C 4种有效成分含量,结合主成分分析对不同品种淫羊藿进行质量分析与评价,并考察淫羊藿对大鼠肾阳虚保护作用。将实验动物随机分为阳虚模型对照组(模型组)、金匮肾气丸干预组(阳性组)、淫羊藿低(125 mg/kg)、中(250 mg/kg)、高(500 mg/kg)剂量组。四周后测定肾阳虚大鼠血清中尿酸(UA)、肌酐(CRE)和尿素氮(BUN)。主成分分析结果显示,主成分1淫羊藿苷、朝藿定C和主成分2朝藿定B是影响样品质量评价的主要因子。12批淫羊藿药材整体质量较好。药理学结果显示,与空白组相比,大鼠血清中UA、CRE和BUN含量显著降低。上述结果表明:本方法同时测定淫羊藿黄酮类4种有效成分,为建立淫羊藿质量评价提供参考;淫羊藿对氢化可的松诱导的肾阳虚具有明显的保护作用,具有量效关系。

Principal component analysis of active flavonoid compounds from different species of Epimedium and its protective effect on kidney-yang deficiency rats

The flavonoids of Epimedium,namely icariin,epimedin A,epimedin B and epimedin C,were determined by HPLC.Combined with principal component analysis(PCA),the quality of different species of Epimedium was analyzed and evaluated.Moreover,the protective effect of Epimedium on kidney-yang deficiency in rats was also investigated.The experimental animals were randomly divided into yang deficiency model control group(model group),Jinkui Shenqi pill intervention group(positive group),Epimedium low(125 mg/kg),medium(250 mg/kg),high(500 mg/kg)dose group.Four weeks later,uric acid(UA),creatinine(CRE)and urea nitrogen(BUN)in serum of rats with kidney-yang deficiency were measured.The results of principal component analysis showed that principal component 1 icariin,epimedin C and principal component 2 epimedin B were the main factors affecting the quality evaluation of samples.The 12 batches of Epimedium medicinal materials were of good overall quality.The pharmacological results showed that compared with the blank group,the levels of UA,CRE and BUN in rat serum were significantly reduced.The above results indicated that the method measured four effective constituents of Epimedium flavonoids in the meantime,which provided a reference for establishing quality evaluation system of Epimedium;Epimedium has obvious protective effect on hydrocortisone-induced kidney-yang deficiency and has a dose-effect relationship.