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| 诱变选育脂肪酶高产菌株及其酶学性质 | |
| 引用本文： | 邓欣,方真,张帆,龙运多,曾虹燕.诱变选育脂肪酶高产菌株及其酶学性质[J].天然产物研究与开发,2011,23(3):512-516. |
| 作者姓名： | 邓欣 方真 张帆 龙运多 曾虹燕 |
| 作者单位： | 1. 中国科学院西双版纳热带植物园昆明分部生物能源组,昆明,650223 2. 中国科学院西双版纳热带植物园昆明分部生物能源组,昆明,650223;湘潭大学化工学院,湘潭,411105 3. 湘潭大学化工学院,湘潭,411105 |
| 基金项目： | 国家高技术研究发展计划资助项目，中国科学院知识创新工程重要方向性项目 |
| 摘 要： | 面包干酵母(Saccharomyces cerevisiae)为出发菌株,对其进行紫外和微波复合诱变,得高产突变菌株DX213,高产突变菌株酶活力为635 U/mL,为出发菌株的1.69倍。菌株富集培养5代,遗传性状稳定。DX213菌株的最优产脂肪酶条件为:培养温度30℃和培养液pH 7.5。酶学性质研究表明:脂肪酶的最适温度40℃、最适pH为7.5、脂肪酶在40℃以下稳定。Fe3+离子对脂肪酶有激活效应,当Fe3+离子浓度为0.03 g/mL时,脂肪酶酶活力高达720 U/mL。 |
| 关 键 词： | 微波 菌株 活性 面包干酵母 诱变 |
Mutation Breeding of High-yield Lipase Strain and Properties of Lipase |
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| DENG Xin,FANG Zhen,ZHANG Fan,LONG Yun-duo,ZENG Hong-yan.Mutation Breeding of High-yield Lipase Strain and Properties of Lipase[J].Natural Product Research and Development,2011,23(3):512-516. | |
| Authors: | DENG Xin FANG Zhen ZHANG Fan LONG Yun-duo ZENG Hong-yan |
| Institution: | DENG Xin1,FANG Zhen1,ZHANG Fan1,2,LONG Yun-duo1,ZENG Hong-yan2 1Biomass group,Xishuangbanna tropical botanical garden,Chinese academy of sciences,Kunming 650223,China,2School of chemical engineering,Xiangtan University,Xiangtan 411105 |
| Abstract: | The mutation strain DX213 was obtained by UV and microwave treatment from Saccharomyces cerevisiae.Its enzymatic activity was 635U/mL,enhanced by 69%.The genetic characters were stable after enrichment culture for five generations.The optimal conditions for producing lipase were culture temperature of 30 ℃ and pH of 7.5.Enzymatic properties were studied and found that the optimal temperature and pH for the lipase were 40 ℃ and 7.5,respectively.The enzymatic activity kept stable below 40 ℃.Fe3+ had an activating effect on lipase.The enzymatic activity went up to 720 U/mL when the Fe3+ concentration was 0.03 g/mL. |
| Keywords: | microwave strain activity Saccharomyces cerevisiae mutation |
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