| 高分子膜载体固定化木瓜蛋白酶 |
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| 引用本文： | 夏葵,曾虹燕,姜和,王亚举,廖凯波.高分子膜载体固定化木瓜蛋白酶[J].天然产物研究与开发,2010,22(1). |
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| 作者姓名： | 夏葵 曾虹燕 姜和 王亚举 廖凯波 |
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| 作者单位： | 湘潭大学化工学院生物技术研究所,湘潭,411105 |
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| 摘 要： | 在浸润条件下,以0.5%(v/v)戊二醛交联的高分子膜尼龙载体固定化木瓜蛋白酶。对固定化条件进行了优化,比较了固定化酶与游离酶的酶学参数。结果表明,4℃、pH6.0条件下,将膜载体浸润于2mg/mL酶液中5h,固定化酶活为303.4U/g。固定化酶最适反应pH为6.0～7.0,最适反应温度为65℃。其pH稳定性、热稳定性均比游离酶高。
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| 关 键 词： | 浸润 尼龙膜 木瓜蛋白酶 固定化 |
Papain Immobilization by Macromolecule Membrane Carrier |
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| XIA Kui,ZENG Hong-Yan,JIANG He,WANG Ya-Ju,LIAO Kai-Bo.Papain Immobilization by Macromolecule Membrane Carrier[J].Natural Product Research and Development,2010,22(1). |
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| Authors: | XIA Kui ZENG Hong-Yan JIANG He WANG Ya-Ju LIAO Kai-Bo |
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| Institution: | XIA Kui,ZENG Hong-Yan,JIANG He,WANG Ya-Ju,LIAO Kai-Bo School of Chemical Engineering,Xiangtan University,Xiangtan 411105,Hunan |
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| Abstract: | Papain was immobilized on 0.5% (v/v) glutaraldehyde cross-linked nylon membrane by soaking method.The optimum immobilized conditions and the characteristics of free and immobilized papain were determined.The results showed that in pH 6.0,the carder was soaked in 2 mg/mL papain solution at 4℃ for 5 h,the activity of immobilized papain 303.4U/g.The pH and temperature of optimum reaction of immobilized papain were 6.0-7.0 and 65℃.The pH and thermal stabilities of immobilized papain were higher than those of free papain. |
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| Keywords: | soaking nylon membrane papain immobilization |
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