| 鞘内注射M受体和GDNF反义寡脱氧核苷酸抑制吗啡戒断大鼠蓝斑c—Fos表达 |
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| 摘 要： |
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| 关 键 词： | 反义寡脱氧核苷酸 鞘内注射 GDNF Fos 表达 大鼠 戒断 胶质细胞源性神经营养因子 蓝斑 M受体 抑制 吗啡依赖 神经元 乙酰胆碱 免疫组化 cell 酸处理 LC 毒蕈碱 适应性 激活 脊髓 |
Intrathecal injection of muscarinic receptors or GDNF antisense oligonucleotides inhibits the increase of c-Fos expression in locus coeruleus of morphine-withdrawal rats] |
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| Authors: | Hui Fen Liu Wen Hua Zhou Shuai En Tang Miao Jun Lai Sheng Liu Wei Sheng Chen Guo Dong Yang |
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| Institution: | Ningbo Institute of Microcirculation & Henbane, Ningbo Aaddiction Research and Treatment Center, Ningbo 315010. lihufen@163.com |
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| Abstract: | The antisense approach and immunohistochemistry were used to study the effects of different muscarinic receptor (M) subtypes and glial cell derived neurotrophic factor (GDNF) on the scores of morphine-withdrawal syndrome and the expression of c-Fos in locus coeruleus (LC). Intrathecal injection of M2 receptor antisense oligonucleotides (M2AS-oligo) or GDNF antisense oligonucleotides (GDNFAS-oligo) decreased the scores of morphine withdrawal syndrome. The expression of c-Fos positive neurons in the LC increased in morphine-dependent rats and increased to a greater extent after the injection of naloxone (4mg/kg, ip) in morphine dependent rats. Intrathecal injection of M2AS-oligo or GDNFAS-oligo inhibited the increase of c-Fos expression in LC during morphine withdrawal, but there was no effect in case of M1AS-oligo. The results suggest that M2 receptor of spinal cord mediates the neural activation of LC during morphine withdrawal. And the interaction between neurons and glial cells may be involved in the ascending activation process. |
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