| Class I patatin基因5‘侧翼区驱动的GUS基因在马铃薯块茎中专一性表达 |
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| 引用本文： | 宋东光,周健,等.Class I patatin基因5‘侧翼区驱动的GUS基因在马铃薯块茎中专一性表达[J].实验生物学报,2001,34(1):5-10. |
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| 作者姓名： | 宋东光 周健 |
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| 作者单位： | [1]复旦大学遗传学研究所、遗传学和遗传工程国家重点实验室，上海200433 [2]复旦大学遗传学研究所、遗传学和遗传工 |
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| 摘 要： |
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| 关 键 词： | 马铃薯 块茎 表达 Class I patatin基因 GUS基因 |
1.4 kb 5' flanking region of class I patatin directs tuber-specific gus expression in potato (Solanum tuberosum L.)] |
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| D G Song,J Zhou,D Q Huang,H Ma,J F Situ,G Q Wang,X M Wang.1.4 kb 5' flanking region of class I patatin directs tuber-specific gus expression in potato (Solanum tuberosum L.)][J].Acta Biologiae Experimentalis Sinica,2001,34(1):5-10. |
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| Authors: | D G Song J Zhou D Q Huang H Ma J F Situ G Q Wang X M Wang |
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| Institution: | State Key Laboratory of Genetics and Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433. |
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| Abstract: | Binary vectors pPATIs (with partial signal sequence) and pPATI (without signal sequence) were constructed by fusing 1.4 kb 5' flanking regions of Class I patatin gene with GUS. Transient GUS expression was observed in in vitro tuber slices bombarded with pPATI. These constructs were then introduced into potato (cv. Desiree) via Agrobacterium tumefaciens transformation. Transgenic potato plants were confirmed by X-Gluc staining and PCR. Using in vitro tuberization system, GUS activities were assayed by fluorescence. It was shown that, in plants transformed with PATI-GUS, GUS specific activities were about 10-20 fold higher in tubers than in stems. Increased sucrose concentration could not induce PATI-GUS expression, but light enhanced PATI-GUS expression in cultured shoots. |
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