白星花金龟对寄主植物挥发物的电生理反应

目的]白星花金龟Protaetia brevitarsis是我国经济上重要的农业害虫.本研究拟鉴定苹果、葡萄和玉米3种果实在两种状态(健康与受白星花金龟成虫为害)下的挥发物中能引起白星花金龟雌成虫触角电生理反应的挥发物成分.方法]采用动态顶空吸附法收集苹果、葡萄和玉米3种果实的挥发物,用气相色谱-触角电位联用技术(...

Electrophysiological responses of Protaetia brevitarsis (Coleoptera: Scarabaeidae) to host plant volatiles

【Aim】 Protaetia brevitarsis is an economically important agricultural insect pest in China. This study aims to identify volatile compounds from apple, grape and corn under two states (healthy and damaged by P. brevitarsis adults) that elicit electrophysiological responses in antennae of female adults of P. brevitarsis. 【Methods】 Fruit volatiles from three fruits, apple, grape and corn, were collected by dynamic headspace adsorption technique. The active compounds in fruit volatile samples were screened by gas chromatography-electroantennogram detection (GC-EAD) technique and further identified by gas chromatography-mass spectrometry (GC-MS) analysis. All EAD-active compounds were quantitated by internal standard method. The active volatile compounds released by healthy and P. brevitarsis damaged fruits were evaluated by principal component analysis (PCA) and cluster analysis (CA). 【Results】Twenty three, 23 and 25 volatile compounds from apple, grape and corn, respectively, were found to be able to trigger EAD responses in female adults of P. brevitarsis. The volatile compounds in six treatments were divided into five groups by CA based on PC1 and PC2, which originated from healthy apple, damaged apple, healthy grape+damaged grape, healthy corn and damaged corn, respectively. There existed apparent differences in the main volatile components among the three fruits. As compared to heathy fruits, damaged fruits released significantly lower amounts of seven esters (2-methylbutyl acetate, propyl 2-methylbutyrate, amyl acetate, methyl hexanoate, butyl butyrate, hexyl acetate and hexyl isovalerate), but significantly greater amounts of three alcohols (2-methylpropanol, 2-methylbutanol and cis-3-hexenol). 【Conclusion】 The volatile compounds from the three fruits that trigger significant EAD responses of adult P. brevitarsis are mainly alcohols and esters. Damage by P. brevitarsis causes significant increase in contents of most alcohols and decrease in contents of esters. The attractiveness of the 58 EAD-active compounds identified in this study needs to be assayed by further laboratory and field experiments.