| 中华蜜蜂和意大利蜜蜂蜂毒前溶血肽原蛋白cDNA的克隆与序列分析 |
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| 引用本文： | 施婉君,张素方,张传溪,程家安.中华蜜蜂和意大利蜜蜂蜂毒前溶血肽原蛋白cDNA的克隆与序列分析[J].昆虫学报,2003,46(2):254-258. |
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| 作者姓名： | 施婉君 张素方 张传溪 程家安 |
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| 作者单位： | 浙江大学应用昆虫学研究所,杭州,310029 |
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| 基金项目： | 国家自然科学基金资助项目(30271008) |
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| 摘 要： | 分别从中华蜜蜂Apis cerana cerana和意大利蜜蜂Apis mellifera工蜂毒腺中抽提总RNA，通过RT-PCR方法扩增,各得到了蜂毒前溶血肽原蛋白的cDNA,再将扩增产物克隆到pGEM-Teasy载体上,进行测序和序列分析。结果表明，所扩增到的这两个片段长度均为213 bp，均为编码蜂毒前溶血肽原的cDNA，并分别推导出两者所编码的氨基酸序列。经序列比较，中华蜜蜂前溶血肽原与意大利蜜蜂、印度蜜蜂Apis cerana indica前溶血肽原的同源性都为97%。所报道的中华蜜蜂蜂毒前溶血肽原的核苷酸序列的GenBank登录号为AF487907。
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| 关 键 词： | 中华蜜蜂 意大利蜜蜂 蜂毒前溶血肽原 克隆 序列分析 |
| 文章编号： | 0454-6296(2003)02-0254-05 |
| 修稿时间： | 2002年3月14日 |
Cloning and sequencing of cDNA encoding prepromelittin in Apis cerana cerana and Apis mellifera |
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| SHI Wan-Jun,ZHANG Su-Fang,ZHANG Chuan-Xi,CHENG Jia-An.Cloning and sequencing of cDNA encoding prepromelittin in Apis cerana cerana and Apis mellifera[J].Acta Entomologica Sinica,2003,46(2):254-258. |
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| Authors: | SHI Wan-Jun ZHANG Su-Fang ZHANG Chuan-Xi CHENG Jia-An |
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| Institution: | SHI Wan-Jun,ZHANG Su-Fang,ZHANG Chuan-Xi,CHENG Jia-An* |
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| Abstract: | Two cDNA fragments encoding prepromelittin were amplified by RT-PCR from the total RNA from the venom glands of the worker honeybees, Apis cerana cerana and A. mellifera. The PCR products were ligated into pGEM-Teasy vector. The results of sequencing showed that the ORFs coding for prepromelittin were all 213 bp in length. The amino acid sequence of A. cerana cerana prepromelittin shared 97% homologies with that of A. mellifera prepromelittin and A. cerana indica prepromelittin. The nucleotide sequence reported here was submitted to GenBank with accession no. AF487907. |
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| Keywords: | Apis cerana cerana Apis mellifera prepromelittin clone sequence analysis |
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