四种鬼臼毒素类似物对美洲大蠊腹神经索动作电位的作用

为了解鬼臼毒素对昆虫神经系统的影响，采用胞外记录电生理方法观察了4种鬼臼毒素类似物对美洲大蠊Periplaneta americana 中枢神经自发放动作电位的影响。结果显示: 在0.1 mmol/L的浓度下，4种物质对美洲大蠊中枢神经自发放动作电位的作用不同。脱氧鬼臼毒素可完全抑制美洲大蠊中枢神经自发放动作电位的产生，其抑制作用可逆；β-阿朴苦鬼臼毒素对美洲大蠊中枢神经自发放动作电位的作用是先兴奋后抑制；鬼臼毒素对美洲大蠊中枢神经自发放动作电位有抑制作用,其抑制作用不完全；4′-去甲鬼臼毒素对大蠊中枢神经自发放动作电位无影响。结果提示,4种鬼臼毒素类似物对昆虫中枢神经自发放动作电位的影响方式与其结构官能团密切相关。

Effects of four podophyllotoxin analogues on spontaneous action potentials of the ventral nerve cord of Periplaneta americana (Blattaria: Blattidae)

The effects of four podophyllotoxin analogues on spontaneous action potentials of the ventral nerve cord of Periplaneta americana were observed by electrophysiology technique. The results showed that at the concertration of 0.1 mmol/L, the four compounds showed different effects on spontaneous action potentials. Dexoypodophyllotoxin exerted irreversible inhibitory effect. β-apopicropodophyllotoxin first exerted excitation and then showed inhibitory effects. Podophyllotoxin showed inhibitory effect, while 4′-demethylpodophyllotoxin did not. The results suggest that the effects of podophyllotoxin analogues on spontaneous action potentials are different according to their structural varieties.