Resistance to deglycosylation by ammonia of IgA1 O-glycopeptides: implications for the beta-elimination of O-glycans linked to serine and threonine |
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Authors: | Tarelli Edward |
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Institution: | Medical Biomics Centre, St Georges University of London, Cranmer Terrace, London SW17 0RE, UK. tarelli@sgul.ac.uk |
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Abstract: | Pools of O-glycopeptides (and their deglycosylated analogues) derived from trypsin-digested normal human serum IgA1 have been treated with ammonia under conditions reported to result in complete liberation of O-glycans linked to serine and threonine residues in glycopeptides and glycoproteins. MALDI-TOF MS analysis has revealed that only one of the six glycosylated sites is susceptible to beta-elimination under these conditions. It is likely that resistance to beta-elimination is due to very close proximity of proline to the glycosylated serine or threonine residues. Preliminary results using 0.1M NaOH (instead of ammonia) to perform beta-elimination indicated that there was also selective de-O-glycosylation with this reagent, however, these results were complicated by the concomitant hydrolysis of the peptide bonds. These findings may have implications for similarly O-glycosylated peptides and proteins and possibly for other chemical methods that are used to carry out beta-eliminations of O-glycans. |
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Keywords: | GalNAc N-acetylgalactosamine Gal galactose NeuAc N-acetylneuraminic acid MALDI-TOF MS matrix assisted laser desorption-time of flight mass spectrometry THAP 2 4 6-trihydroxyacetophenone |
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