| 一种新的cDNA 末端快速扩增获取全长cDNA的方法 |
| |
| 引用本文： | 邱为民,张思仲,武辉,张戈,肖翠英QIU Wei-min,ZHIANG Si-zhong,WU Hui,ZHANG Ge,XIAO Cui-ying.一种新的cDNA 末端快速扩增获取全长cDNA的方法[J].遗传,2001,23(5):480-481. |
| |
| 作者姓名： | 邱为民 张思仲 武辉 张戈 肖翠英QIU Wei-min ZHIANG Si-zhong WU Hui ZHANG Ge XIAO Cui-ying |
| |
| 作者单位： | 四川大学华西医院医学遗传研究室,都 610041
Department of Medical Genetics,First University Hospital,West China Medical Center,Sichuan University,Chengdu 610041,P.R.China |
| |
| 摘 要： | 为克隆精子发生相关基因的全长cDNA,根据mRNA差异显示获得的ESTs设计引物,利用一种新的cDNA末端快速扩增方法(SMARTRACE)扩增该EST的5′末端,并进行克隆测序,与mRNA差异显示获得ESTs拼接后,获得了三个新的全长cDNA.结果表明,SMARTRACE是一种简便、有效的克隆cDNA5′末端未知序列的技术.
Abstract:To clone the full-length cDNAs of genes related to spermatogenesis,ESTs obtained by mRNA differential display were used to design gene-specific primer.Then SMART RACE was performed to obtain the 5′ region of these ESTs.After cloning,sequencing and splicing with ESTs obtained by mRNA differential display,three full-length cDNAs were obtained.The results indicate that SMART RACE is a simple and an effective technique for cloning 5′-end unknown sequence of gene.
|
| 关 键 词： | SMART RACE spermatogenesis-related gene 精子发生相关基因 Key words cDNA 末端快速扩增 |
A Novel Method of Rapid Amplification of cDNA Ends for Obtaining the Full-Length cDNA |
| |
| QIU Wei-min,ZHIANG Si-zhong,WU Hui,ZHANG Ge,XIAO Cui-ying.A Novel Method of Rapid Amplification of cDNA Ends for Obtaining the Full-Length cDNA[J].Hereditas,2001,23(5):480-481. |
| |
| Authors: | QIU Wei-min ZHIANG Si-zhong WU Hui ZHANG Ge XIAO Cui-ying |
| |
| Abstract: | |
| |
| Keywords: | |
|
| 点击此处可从《遗传》浏览原始摘要信息 |
| 点击此处可从《遗传》下载免费的PDF全文 |
|
