基于大鼠在体肠灌流模型研究白及有效部位在肠道的可吸收及代谢成分

为了考查白及有效部位在肠道的可吸收成分及其代谢特征。基于在体肠灌流模型,采用超高效液相色谱-四极杆-飞行时间质谱仪(UPLC-Q-TOF/MS)对收集到的健康SD大鼠循环肠灌流液、血清、胆汁进行分析检测,并结合对照品、质谱碎片信息和Masslynx V4.1工作站中的Single Mass Analysis功能,初步推测吸收和代谢产物的结构式。在大鼠血清和胆汁中,初步鉴定出1,4-二4-(葡萄糖氧)苄基]-2-异丁基苹果酸、4-(葡萄糖氧)苄基]-2-异丁基苹果酸酯、α-异丁基苹果酸酯原型产物。在大鼠循环肠灌流液、血清和胆汁中,共鉴定出4-(葡萄糖氧)苄基]-2-异丁基苹果酸酯的脱糖后硫酸化代谢产物和二氢菲5的葡萄糖醛酸化代谢产物,其代谢产物主要生水解和葡萄糖醛酸化反应。该方法初步探究了白及有效部位在大鼠循环肠灌流液中可吸收成分和代谢特征,为阐释白及药材的药效物质基础提供实验依据。

Study on the absorbable components and metabolism of Bletilla striata effective parts in the intestine based on rat intestinal perfusion model

In order to examine the absorbable components of the Bletilla striata effective parts in the intestine and their metabolic characteristics.Based on the intestine perfusion model,the collected intestinal perfusate,serum and bile of healthy SD rats were analyzed by UPLC-Q-TOF/MS.And combined with the reference substance,MS fragment information and the Single Mass Analysis function in the Masslynx V4.1 workstation,preliminarily speculated the structure of the absorption and metabolites.militarine,gymnoside I andα-isobutylmalic acid were preliminarily identified in serum and bile of rats.A total of gymnoside I and dihydrophenanthrene 5 metabolites were identified in rat circulating intestinal perfusion fluid,serum and bile.The method preliminarily explored the absorbable components and metabolic characteristics of Bletilla striata effective parts in the circulating intestinal perfusate of rats,and provided experimental basis for explaining the pharmacodynamic basis of white and medicinal materials.