| 宁前胡内生真菌Fusarium tricinctum次生代谢产物研究 |
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| 引用本文： | 刘丛彬,刘海涛,杨家欣,刘劲松,王刚,王国凯.宁前胡内生真菌Fusarium tricinctum次生代谢产物研究[J].天然产物研究与开发,2019(9):1580-1584. |
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| 作者姓名： | 刘丛彬 刘海涛 杨家欣 刘劲松 王刚 王国凯 |
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| 作者单位： | 安徽中医药大学药学院现代中药安徽省工程技术中心;中国医药大学附设医院医学研究部 |
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| 基金项目： | 安徽省高校优秀青年人才支持计划重点项目(gxyqZD 2019035);安徽省高校科研创新平台团队-中药饮片产地加工与炮制一体化关键技术研究创新团队(皖教秘科[2015]49号) |
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| 摘 要： | 为研究宁前胡内生真菌Fusariumtricinctum次生代谢产物,采用液体发酵法发酵内生真菌,利用硅胶、凝胶、MPLC、制备液相等多种方法分离获得13个单体化合物。利用波谱方法分别鉴定为cyclo-(L-pro-L-pro)(1)、cyclo-(S-pro-S-leu)(2)、cyclo-(L-phe-L-phe)(3)、cyclo-(D-pro-L-phe)(4)、cyclo-(L-pro-L-phe)(5)、cyclo-(D-pro-L-leu)(6)、cyclo-(S-pro-S-leu)(7)、cyclo-D-(4-hydroxyprolinyl)]-(L)-leucine(8)、cyclo-L-(4-hydroxyprolinyl)]-(L)-leucine(9)、cyclo-(trans-4-hydroxy-L-prolyl-L-phenylalanine)(10)、cyclo-(D-cis-hyp-L-phe)(11)、苯甲酸苄酯(12)和苯乙酸(13),所有化合物均首次从内生真菌F.tricinctum中分离得到。体外抗肿瘤活性显示,化合物4、6、7对CAR、CAL27、SCC-4、SCC-9、HSC-3五种口腔癌肿瘤细胞有一定的细胞毒活性。
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| 关 键 词： | 内生真菌 宁前胡 FUSARIUM tricinctum 次生代谢产物 抗肿瘤活性 |
Secondary metabolites of endophytic fungus Fusarium tricinctum from Ningguo Peucedanum praeruptorum Dunn. |
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| LIU Cong-bin,LIU Hai-tao,YANG Jai-sing,LIU Jin-song,WANG Gang,WANG Guo-kai.Secondary metabolites of endophytic fungus Fusarium tricinctum from Ningguo Peucedanum praeruptorum Dunn.[J].Natural Product Research and Development,2019(9):1580-1584. |
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| Authors: | LIU Cong-bin LIU Hai-tao YANG Jai-sing LIU Jin-song WANG Gang WANG Guo-kai |
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| Institution: | (School of Pharmacy,Anhui University of Chinese Medicine & Anhui Engineering Research Center of Modernized Chinese Material,Hefei 230012,China;Department of Medical Research,China Medical University Hospital,China Medical University,Taichung 40447,China) |
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| Abstract: | To study the secondary metabolites of endophytic fungus Fusarium tricinctum from Ningguo Peucedanum praeruptorum Dunn.Compounds were isolated from the EtOAc extract by chromatography technology and their structures were elucidated on the basis of comprehensive spectroscopic analysis.Thirteen compounds were isolated and their structures were identified as cyclo-( L -pro- L -pro)( 1 ),cyclo-( S -pro- S -leu)( 2 ),cyclo-( L -phe- L -phe)( 3),cyclo-( D -pro- L -phe)(4),cyclo-( L -pro- L -phe)(5),cyclo-( D -pro- L -leu)(6),cyclo-( S -pro- S -leu)(7 ),cyclo- D -(4-hydroxyprolinyl)]-( L )-leucine ( 8 ),cyclo- L -(4-hydroxyprolinyl)]-( L )-leucine (9),cyclo-( trans -4-hydroxy- L -prolyl- L -phenylalanine)(10),cyclo-( D -cis-hyp- L -phe)( 11),benzyl benzoate (12),phenylacetic acid (13 ).All these compounds were isolated from Fusarium tricinctum for the first time.The cytotoxicity assay showed that compounds 4,6 and 7 had moderate cytotoxic activities against CAR,CAL27,SCC-4,SCC-9,and HSC-3. |
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| Keywords: | endophytic fungus Ningguo Peucedanum praeruptorum Dunn Fusarium tricinctum secondary metabolites anti-tumor |
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