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| 拟南芥赤霉素受体AtGID1a原核表达条件的优化研究 | |
| 引用本文： | 周敏,赵卓亚,徐文忠,肖浪涛,王若仲.拟南芥赤霉素受体AtGID1a原核表达条件的优化研究[J].激光生物学报,2011,20(1):103-107. |
| 作者姓名： | 周敏 赵卓亚 徐文忠 肖浪涛 王若仲 |
| 作者单位： | 1. 湖南农业大学植物激素与生长发育湖南省重点实验室,湖南,长沙,410128;中国科学院植物研究所光合作用与环境分子生理学重点实验室,北京,100093 2. 中国科学院植物研究所光合作用与环境分子生理学重点实验室,北京,100093 3. 湖南农业大学植物激素与生长发育湖南省重点实验室,湖南,长沙,410128 |
| 基金项目： | 湖南省自然科学基金，教育部新世纪优秀人才支持计划项目，湖南省科研条件创新专项 |
| 摘 要： | 运用均匀设计法,对影响Rossetta(DE3)/pGEX-KG/AtGID1a表达水平的因素进行了优化,摸索出了对可溶性蛋白进行诱导表达的最优条件,即蛋白胨16 g/L,酵母粉5 g/L,葡萄糖4g/L,IPTG终浓度0.1 mmol/L,诱导温度23 ℃,IPTG诱导时间4 h.在此条件下,目的蛋白的平均密度从0.... |
| 关 键 词： | pGEX-AtGID1a 均匀设计 偏最小二乘回归 优化表达 |
Optimization for Prokaryotic Expression Conditions of Gibberellins Receptor AtGID1 a in Arabidopsis |
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| ZHOU Min,ZHAO Zhuo-ya,XU Wen-zhong,XIAO Lang-tao,WANG Ruo-zhong.Optimization for Prokaryotic Expression Conditions of Gibberellins Receptor AtGID1 a in Arabidopsis[J].ACTA Laser Biology Sinica,2011,20(1):103-107. | |
| Authors: | ZHOU Min ZHAO Zhuo-ya XU Wen-zhong XIAO Lang-tao WANG Ruo-zhong |
| Institution: | ZHOU Min~(1,2),ZHAO Zhuo-ya~2,XU Wen-zhong~2,XIAO Lang-tao~1,WANG Ruo-zhong~(1*) (1.Hunan Provincial Key Laboratory of Phytohormones and Growth Development,Hunan Agricultural University,Changsha 410128,Hunan,China,2.Key Laboratory of Photosynthesis and Environmental Molecular Physiology,the Chinese Academy of Sciences,Beijing 100093,China) |
| Abstract: | The factors that influenced expression level of Rossetta（ DE3 ）/pGEX-KG/AtG1Dla were optimized using unifotm design. The optimization induction condition for the solubility protein expression were investigated. The results were as followed： 16 g/L peptone, 5 g/L yeast powder, 4 g/L glucose, 0.1 mmol/L IPTG, 23℃ induction temperature and 4 h induction time. The average density of interest protein had increased from 0. 042 to 0. 064. The optimization rate was 52 %. |
| Keywords: | pGEX-AtGID1a |
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