Genetic variation of Calycophyllum spruceanum in the Peruvian Amazon Basin, revealed by amplified fragment length polymorphism (AFLP) analysis |
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Authors: | J R RUSSELL J C WEBER A BOOTH W POWELL & C SOTELO-MONTES† and I K DAWSON |
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Institution: | Department of Cell &Molecular Genetics, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK,,;International Centre for Research in Agroforestry (Regional Programme), c/o Instituto Nacional de Investigación Agraria-Centro Forestal, Carretera Federico Basadre km 4.2, Apartado Postal 617, Pucallpa, Peru,,;International Centre for Research in Agroforestry (Headquarters), United Nations Avenue, PO Box 30677, Nairobi, Kenya |
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Abstract: | An understanding of the level, structure and origin of genetic variation within and among populations of tropical trees is essential for devising optimum management strategies for their sustainable utilization and conservation. Here, amplified fragment length polymorphism (AFLP) analysis was used to partition genetic variation within and among nine populations of the predominantly riverine tree, Calycophyllum spruceanum , sampled across a wide geographical range along river tributaries of the Peruvian Amazon Basin. Analysis of molecular variance ( AMOVA ) employed 65 AFLP markers and revealed most variation among individuals within populations (91%), although variation among populations was highly significant ( P < 0.001). Calculation of genetic distances and nested AMOVA indicated a degree of structuring among populations based on geographical proximity, although clustering did not depend on geographical distance alone. No firm evidence was obtained for unidirectional seed dispersal by water playing an important role in determining genetic structure over the geographical range sampled. Implications of data for optimising genetic management of the species are discussed and areas for further study identified. |
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Keywords: | amplified fragment length polymorphisms (AFLP) Calycophyllum spruceanum conservation Peruvian Amazon tropical pioneer tree water-mediated seed dispersal |
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