宽皮柑橘品种的胚性愈伤组织诱导

以宽皮橘3个品种的幼嫩胚珠和8个品种成熟果实中未发育胚珠为试材,采用EME(MT 500mg/L麦芽浸出物)、MKT(EME 10mg/L KT)、MGS(EME 1mg/L GA3 40mg/L硫酸腺嘌呤)和MDB (EME 0.01mg/L 2,4-D 0.1mg/L BA)4种培养基进行胚性愈伤组织诱导,结果表明EME与MKT培养基配合使用有利于从幼嫩胚珠获得胚性愈伤组织;MGS比MDB更有利于成熟果未发育胚珠胚性愈伤组织的诱导;暗培养有利于此诱导过程。经两种途径获得的胚性愈伤组织,染色体数目稳定,均为二倍体2n=2x=18。

INDUCTION OF EMBRYOGENIC CALLUS OF LOOSE SKIN MANDARINS

Young ovules from 3 cultivars and undeveloped ovules in mature fruits from 8 cultivars of loose skin mandarin of Citrus were cultured on 4 different media respectively to induce embryo-genie calli. Results showed that the combination of EME(MT 500mg/L malt extract) and MKT (EME 10mg/L KT) media performed well in the induction of embryogenic calli from young ovules; MGS(EME 1mg/L GA3 40mg/L sul- fate adenine)medium was better than MDB(MT 0.01mg/L 2,4-D 0. 1mg/L BA)medium in inducing calli from the undeveloped ovules, and the darkness was conducive to the induction of embryogenic calli. There was no chromosome number variation in the induced calli. All of the examined cells were diploid with 2n = 2x = 18 chromosomes.