栽培一粒小麦3AA30中抗白粉病基因的鉴定及分子标记定位

栽培一粒小麦是普通小麦的近缘种,遗传多样性丰富,蕴含丰富的抗病基因,是小麦抗病性改良的重要资源。本文对栽培一粒小麦抗白粉病材料3AA30的抗白粉病基因进行了遗传分析和分子标记定位。结果表明,3AA30中含有一个隐性抗白粉病基因,暂命名为ml3AA30,找到了5个与该基因连锁的SSR分子标记Xgwm6、Xcfd39、Xcfa2185、Xcfa2141、Xcfa2155及2个STS标记Xmag2170、Xmag1491,并构建了ml3AA30的遗传连锁图,将该基因定位在小麦5A染色体长臂上。本研究为小麦抗病育种提供了新的抗源材料。

Identification and molecular mapping of the powdery mildew resistance gene in einkorn wheat (Triticum monococcum L.) accession 3AA30

The einkorn wheat contains abundant resistance genes and is one of important resources for common wheat cultivar improvement. An einkorn wheat accession 3AA30 was proved to be resistant to wheat powdery mildew in Beijing. Genetic analysis showed that 3AA30 carries a recessive resistance gene, temporally designated ml3AA30. Five SSR markers (Xgwm6, Xcfd39, Xcfa2185, Xcfa2141, Xcfa2155) and two STS markers (Xmag2170, Xmag1491) on chromosome 5AL of wheat were found linked to ml3AA30 by bulked segregant analysis, and a genetic map of ml3AA30 was established. Based on the above result,powdery mildew resistance gene ml3AA30 was located on the long arm of wheat chromosome 5A. This result provides new materical for wheat powdery mildew resistance breeding program.