YP3：食用菌榆黄蘑中新的植物病毒抑制物蛋白

食用菌中含有多种抗病毒蛋白,可用于植物保护.采用硫酸铵分级沉淀、DEAE-纤维素离子交换层析和SephacrylTMS-200凝胶层析方法,从食用菌榆黄蘑新鲜子实体中提取到一单亚基蛋白,命名为YP3.利用SDS-聚丙烯酰胺不连续凝胶电泳初步估计其分子量大约为27.6 kDa.氨基酸组成分析表明该蛋白非常类似其它的抗植物病毒蛋白,并且几乎不含糖.其N-末端序列为NRDVAACARFIDDFCDTLTP,在GenBank中没有找到同源序列.浓度为0.24 mg/L时蛋白YF3对烟草花叶病毒(TMV 20 mg/L)侵染心叶烟的抑制率为50%.同时还发现YP3对供试的细菌和真菌没有抑制活性,对胃癌细胞株MGC-803、肝癌细胞株SMMC-7721、肺癌细胞株SPC-A1的细胞增殖具有一定的抑制作用,其IC50大约为20 mg/L.

YP3:a Novel Plant Virus Inhibitory Protein from Mushroom Pleurotus citrinopileatus

Many proteins from mushrooms can be used as potential antiviral agents,and in plant protection.An antiviral protein(YP3)was isolated from fruiting bodies of the fungus Pleurotus citrinopileatus with a procedure involving ammonium sulfate precipitation,ion-exchange chromatography on DEAE-cellulose,and gel filtration on SephacrylTMS-200 High Resolution.The molecular weight of protein was determined to be about 27.6 kDa by Sodium dodecyl sulfate -polyacryl-amide gel electrophoresis(SDS-PAGE).The amino acid analysis indicated that it was similar to some other antiviral proteins.The protein was almost not sacchariferous.The sequence of N-terminal of YP3 was NRDVAACARFIDDFCDTLTP,which had no homology with other sequences in GenBank.YP3 at 0.24 mg/L achieved 50.0% inhibition of Tobacco mosaic virus(TMV 20 mg/L)lesions in Nicotiana glutinosa leaves;YP3 is inactive to some bacteria and fungi tested;YP3 inhibited stomach cancer cell line MGC-803,liver cancer cell line SMMC-7721 and lung cancer cell line SPC-Alwith an IC50,of about 20 mg/L.