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| 正交试验优选环草石斛(D.loddigesii Rolfe.)组培苗壮苗生产工艺 | |
| 引用本文： | 吴明开,朱国胜,毛堂芬,刘作易,金家兴,毛秀华.正交试验优选环草石斛(D.loddigesii Rolfe.)组培苗壮苗生产工艺[J].激光生物学报,2008,17(2):265-269. |
| 作者姓名： | 吴明开 朱国胜 毛堂芬 刘作易 金家兴 毛秀华 |
| 作者单位： | 1. 安徽师范大学生命科学学院,安徽,芜湖,241000 2. 贵州省农业生物技术重点试验室,贵州,贵阳,550006;贵州省农科院生物技术研究所,贵州,贵阳,550006 3. 贵州省农科院生物技术研究所,贵州,贵阳,550006 4. 贵州省农业生物技术重点试验室,贵州,贵阳,550006 5. 兴义市吉仁堂药业公司,贵州,兴义,562400 |
| 基金项目： | 贵州省中药现代化科技产业研究开发专项基金 |
| 摘 要： | 采取正交试验设计对环草石斛(Dendrobium loddigesiiRolfe.)组培苗壮苗进行了优化生产工艺研究,以培养基(A)、添加物马铃薯汁液(B)、激素NAA(C)和pH值(D)为因素,以环草石斛组培苗的株高(cm)、茎粗(cm)、叶片数、叶面积(cm2)、根数、根长(cm)、丛生芽数、单株鲜重(g)等各个性状的增加值为考察指标,优选环草石斛组培苗壮苗的生产工艺。其中自己设计了一种改良培养基(WM),结果显示,改良培养基、添加物马铃薯汁液、激素NAA和pH值均对环草石斛组培苗壮苗产生显著影响,其影响程度为培养基>pH>马铃薯汁液>NAA。由此正交试验筛选出环草石斛组培苗壮苗较优生产工艺为A3B3C3D2,即培养基WM,添加马铃薯汁液200 g/L,NAA2 mg/L,pH值5.6。优选出的壮苗工艺经济,简便,苗壮根多且长,适宜工厂化生产。 |
| 关 键 词： | 环草石斛 正交试验 壮苗 优选 |
| 文章编号： | 1007-7146(2008)02-0265-05 |
| 收稿时间： | 2007/5/26 |
| 修稿时间： | 2007年5月26日 |
Optimization to the Seedling Growth of Dendrobium loddigesii Rolfe. in vitro by Orthogonal Design |
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| WU Ming-kai,ZHU Guo-sheng,MAO Tang-fen,LIU Zuo-yi,JIN Jia-xing,MAO Xiu-hua.Optimization to the Seedling Growth of Dendrobium loddigesii Rolfe. in vitro by Orthogonal Design[J].ACTA Laser Biology Sinica,2008,17(2):265-269. | |
| Authors: | WU Ming-kai ZHU Guo-sheng MAO Tang-fen LIU Zuo-yi JIN Jia-xing MAO Xiu-hua |
| Abstract: | Optimization production techniques to the seedling growth of Dendrobium loddigesii Rolfe.in vitro was studied by orthogonal design L9(34),and the results showed that the best seedling technology was A3B3C3D2,namely which was WM medium supplemented with 200 mg/L potato juice and NAA 2 mg/L and the pH 5.6.The influence of factors were highly significant,and the efficiency was as follows: This technology is worthy of medium>pH>potato juice>NAA.This technology is inexpensive,simple and effective,which can be used as experimental basis of seedling growth of Dendrobium loddigesii Rolfe.in factory production. |
| Keywords: | Dendrobium loddigesii Rolfe orthogonal test seedling growth optimization |
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