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| 响应面法优化南方山荷叶中鬼臼毒素的提取工艺 | |
| 引用本文： | 邱子栋,金青浩,郭明全.响应面法优化南方山荷叶中鬼臼毒素的提取工艺[J].植物科学学报,2017,35(5):774-782. |
| 作者姓名： | 邱子栋 金青浩 郭明全 |
| 作者单位： | 1. 中国科学院武汉植物园, 植物种质创新与特色农业重点实验室, 武汉 430074; 2. 中国科学院大学, 北京 100049; 3. 中国科学院上海药物研究所, 中药标准化技术国家工程实验室, 上海 201203; 4. 中国科学院中非联合研究中心, 武汉 430074 |
| 基金项目： | 国家自然科学基金项目（81673580）；中国科学院百人计划资助项目（29Y429291a0129）。 |
| 摘 要： | 鬼臼毒素（Podophyllotoxin）是一种常用的临床天然药物。本研究以鬼臼类药材南方山荷叶（Diphylleia sinensis H.L.Li）为材料，以提取物中鬼臼毒素的含量为控制指标，应用响应面法优化南方山荷叶中鬼臼毒素的提取工艺。采用高效液相色谱法测定提取物中鬼臼毒素的含量，在单因素实验筛选的基础上，选择出对鬼臼毒素提取率影响较大的3个因素：乙醇浓度、提取温度和提取时间，然后利用Design-expert软件设计3因素3水平响应面实验，最终获得鬼臼毒素提取率最高时的提取条件为：乙醇浓度48.18%、提取温度62.66℃、提取时间16.15 h，此时鬼臼毒素的理论提取率可达7.636%。进一步对该条件进行实验验证，3次实验平均值为7.54%，与理论值差异较小，表明该模型拟合良好，实验方法可靠。 |
| 关 键 词： | 南方山荷叶 鬼臼毒素 响应面 提取工艺 |
| 收稿时间： | 2016-12-29 |
Optimization of the extraction process of podophyllotoxin from Diphylleia sinensis H. L. Li by response surface methodology |
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| Qiu Zi-Dong,Jin Qing-Hao,Guo Ming-Quan.Optimization of the extraction process of podophyllotoxin from Diphylleia sinensis H. L. Li by response surface methodology[J].Plant Science Journal,2017,35(5):774-782. | |
| Authors: | Qiu Zi-Dong Jin Qing-Hao Guo Ming-Quan |
| Institution: | 1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China; 3. National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; 4. Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China |
| Abstract: | Podophyllotoxin (PPT) is a frequently used clinical natural drug. In this study, we aimed to optimize the extraction process of PPT from Diphylleia sinensis H. L. Li by response surface methodology (RSM). Based on single factor experiments, three important factors, that is, ethanol concentration, extraction temperature, and extraction time, were selected for further optimization with three-factor/three-level Box-Behnken design. Based on the extraction rate of PPT, the optimal conditions were:ethanol concentration of 48.18%, extraction temperature of 62.66℃, and extraction time of 16.15 h. Under these optimal conditions, PPT yield could reach 7.636%, with the confirmatory experiment reaching 7.54%. Thus, there was no significant difference compared with the theoretical value, verifying the optimized method with RSM in this study. |
| Keywords: | Diphylleia sinensis Podophyllotoxin Response surface methodology Extraction process |
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