十字花科上的链格孢属真菌同工酶凝胶电泳分析

本文报道对从国内外收集到的生于十字花科植物上的3种链格孢属真菌21个菌株进行的10种同工酶的聚丙烯酰胺凝胶电泳分析结果。这10种酶分别是:酯酶(EST)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、多酚氧化酶(PPO)、过氧化氢酶(CAT)、谷氨酸脱氢酶(GDH)、甘露醇脱氢酶(MADH)、乙醇脱氢酶(ADH)、黄嘌呤脱氢酶(XDH)、过氧化物岐化酶(SOD)。对同工酶酶谱资料的聚类分析,在较高的相似性水平上将21个菌株归为3大类群,每一类群所包括的菌株与形态学的鉴定结果完全一致,各菌株的归类关系与其寄主植物、地理来源无关。表明同工酶凝胶电泳在Alternaria种级分类中是一个有用的工具,可以明确地将不同菌株鉴定到种级水平。

ANALYSIS OF ISOZYMES BY PAGE OF ALTERN ARIA SPP FROM CRUCIFERAE

Twenty-one representative isolates of Alternaria belonging to three species were chosen for study from 211 isolates obtained from more than 200 fungal specimens on plant family Cruciferae. Among the 21 isolates, 10 were A. brassicae, 6 were A. brassicicola, 5 were A. tenuis. They were compared by using polyacry-lamide gel electrophoresis of isozyme analysis. The enzymes were esterase (E. C. 3. 1. 1. 1. EST), alkaline phosphatase (E. C. 3. 1. 3. 1. AKP), acid phosphatase (E. C. 3. 1. 3. 2. ACP), polyphenoloxidase (PPO), catalase (CAT), glutamate de-hydrogenase (E. C. 1. 4. 1. 3. GDH), mannitol dehydrogenase (E. C. 1. 1. 1. 67. MADH), alcohol dehydrogenase (E. C. 1. 1. 1. 1. ADH), xanthine dehydrogenase (E. C. 1. 2. 1. 37. XDH), superoxide dismutase (E. C. 1. 1. 5. 11. SOD). Fuzzy cluster of data from isozymes reasonably grouped the isolates into three groups. The grouping of isolates was completely consistent with the morphological taxonomy results, i.e. the isolates in one group standed for one species. The results indicated that banding patterns of isozymes were species-specific and were not related to hosts, substrates and geographical distributions. It revealed that the electrophoresis of isozyme analysis might be useful for distinguishing different species of Alternaria.