|
|||||
|
|
| 26种冬青属植物遗传多样性分析 | |
| 引用本文： | 章建红,高云振,张斌,王正加.26种冬青属植物遗传多样性分析[J].西北植物学报,2011,31(3):504-510. |
| 作者姓名： | 章建红 高云振 张斌 王正加 |
| 作者单位： | 1. 浙江省宁波市农业科学研究院林业研究所,浙江宁波,315040 2. 浙江省三门县林业特产局,浙江三门,317111 3. 海盐县农业经济局林特花卉站,浙江海盐,314300 4. 浙江农林大学,浙江临安,311300 |
| 基金项目： | 宁波市农业与社会发展攻关项目 |
| 摘 要： | 以26种冬青属植物种质资源为研究材料,利用RAPD和AFLP技术对基因组DNA进行扩增,以研究其物种间遗传多样性以及亲缘关系.结果表明:在RAPD分析中,从400条10个碱基的寡核苷酸引物中筛选出反应稳定、扩增性强、重复性好的引物20个,共扩增出312条多态性条带,多态率为95.41%;聚类分析显示26种冬青属植物间,布利奥特夫人枸骨叶冬青和黄果在AFLP分析中,10对选择性引物组合均扩增出了丰富的多态性片段,共扩增出350条谱带,其中336条具有多态性,占95.96%.综合RAPD和AFLP聚类结果,枸骨、无刺枸骨和日拉斯纳尔逊枸骨的亲缘关系较近,钝齿冬青、金宝石钝齿冬青和龟甲冬青三者的亲缘关系较近,可为冬青属植物的杂交育种与种质创新提供理论依据. |
| 关 键 词： | 冬青属 RAPD AFLP 遗传多样性 |
Genetic Diversity of Ilex L.Tree Species |
|
| ZHANG Jian-hong,GAO Yun-zhen,ZHANG Bin,WANG Zheng-jia.Genetic Diversity of Ilex L.Tree Species[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(3):504-510. | |
| Authors: | ZHANG Jian-hong GAO Yun-zhen ZHANG Bin WANG Zheng-jia |
| Institution: | ZHANG Jian-hong1,GAO Yun-zhen2,ZHANG Bin3,WANG Zheng-jia4(1 Ningbo Agriculture Science Academy,Ningbo,Zhejiang 315040,China,2 Sanmen Forestry Specialty Bureau of Zhejiang,Sanmen,Zhejiang 317111,3 Forest Peculiar and Flower Station Bureau of Agricultural Economics of Haiyan,Haiyan,Zhejiang 314300,4 Zhejiang Agriculture and Forestry University,Linan,Zhejiang 311300,China) |
| Abstract: | RAPD and AFLP were used to amplify the genomic DNA of 26 Ilex species to study the species genetic diversity and genetic relationship.The results showed that 20 primers with good reaction stability,strong amplification ability and good reproducibility were screened from 400 oligonucleotide primers with 10 base pairs for RAPD analysis and 312 polymorphic bands were obtained,of which 95.41% were polymorphic.Cluster analysis revealed that the biggest similarity coefficient was between Ilex aquifolium 'Madame B... |
| Keywords: | RAPD AFLP |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |