Immobilization of endo-polygalacturonase from Aspergillus ustus on silica gel |
| |
Authors: | M Narsimha Rao AA Kembhavi A Pant |
| |
Institution: | (1) Division of Biochemical Sciences, National Chemical Laboratory, Pune, 411008, India |
| |
Abstract: | Endo-polygalacturonase from Aspergillus ustus when immobilized on to modified silica gel retained 28% of its original activity. The immobilized enzyme could be re-used through 10 cycles of reaction with almost 90% retention of its original activity. It had increased thermostability over its soluble form: the half-life of the soluble enzyme at 40 °C was less than 10 h whereas the immobilized enzyme retained 82% of its activity after 10 h at 40 °C. Similarly, at 50 °C the half-life of the soluble enzyme was 30 min whereas that of the immobilized enzyme was 5 h. |
| |
Keywords: | Aspergillus ustus endo-polygalacturonase immobilization silica gel |
本文献已被 SpringerLink 等数据库收录! |