子宫内膜癌组织中uPA及Cath-D的表达及意义(英文)

目的:检测子宫内膜癌组织中尿激酶型纤溶酶原激活物(uPA)及组织蛋白酶(Cath-D)的表达并探讨相关性及其临床意义。方法:采用免疫组织化学方法(PV-6000二步法)检测31例子宫内膜癌组织(内膜癌组),17例子宫内膜增生组织(增生组)及10例正常子宫内膜组织(对照组)中uPA及Cath-D的表达,并研究其相关性。结果:1.内膜癌组中uPA和Cath-D的表达均高于增生组及对照组中的表达,差异均有统计学意义(P0.05);在增生组中的表达与对照组差异无统计学意义(P0.05)。2.uPA和Cath-D的阳性表达与子宫内膜癌的临床病理分期、组织学分级及肌层浸润深度有关,差异均具有统计学意义(P0.05)。3.内膜癌组中uPA与Cath-D的表达呈正相关(r=0.673,P0.05)。结论:uPA和Cath-D在子宫内膜癌发生发展及侵袭转移过程中起着协同作用,Cath-D可诱导产生活化的uPA,促进癌细胞的浸润转移,因此,两者的联合检测可有助于成为判断子宫内膜癌的发展及预后的重要指标。

Expression and Clinical Significance of uPA and Cath-D in Endometrial Carcinoma

ABSTRACT Objective:To investigate the expression of urokinase-type plaminogen activator (uPA) and cathepsinD (Cath-D) in endometrial carcinoma and the correlation of the expression with clinical pathological feature of endometrial carcinoma.Methods: Ten cases of normal endometrium specimens, 17 cases was endometrium hyperplasia tissues, and 31 cases of endometrial cancer specimens, were analyzed using immunohistochemistry. The relationships among uPA and Cath-D in the endometrial cancer were analyzed by X2 test, Fish''s exactly test and Pearson test. Results:The positive expression rates of uPA and Cath-D in the endometrial carcinoma tissue were significantly higher than those of the endometria1 hyperplasia tissue and the normal endometrial one''s respectively(P<0.05). There''s no significance between the endometrial hyperplasia and the normal one''s in the expression of uPA and Cath-D. The expression of uPA and Cath-Dvaries according to the disease stage, histological grade and myometrial invasion of endometrial carcinoma (P<0.05). Cath-D positive cases were significantly positively correlated with uPA positive cases (r=0.673, P<0.05).Conclusion: The interaction between uPA and Cath-D plays a coordination role in the invasion and metastasis endometrial carcinoma. Cath-D can promote uPA in the process of invasion and metastasis, so uPA and Cath-D may be important indexes to predict the prognosis of endometrial carcinoma.