Survivin与VEGF在良、恶性胃溃疡组织中的表达及研究

目的:研究凋亡基因生存素(Survivin)及血管内皮生长因子(VEGF)在良、恶性胃溃疡中的表达及二者在溃疡型胃癌中的表达与临床病理特征之间的关系,分析二者在胃癌发生发展中的作用和在胃癌中表达的相关性。方法:应用免疫组化S-P染色检测Survivin及VEGF在良性胃溃疡,胃溃疡伴中-重度不典型增生和溃疡性胃癌中的表达,结合临床病理特征进行相关分析。结果:Survivin及VEGF在良性胃溃疡中的表达率分别为16.2%、24.3%,在胃溃疡伴中-重度不典型中的表达率分别为52.3%、45.5%,在溃疡型胃癌中的表达率分别为71.4%、55.4%,差异具有显著性(P0.01);Survivin和VEGF的表达与溃疡型胃癌的浸润深度、淋巴结转移、TNM分期具有相关性。Survivin和VEGF的表达亦呈正相关。结论:Survivin基因在溃疡型胃癌组织中的表达显著增高,是胃癌演变进程中的重要步骤,过表达Survivin可能提示预后不良。Survivin对胃癌的诊断及预后有潜在的应用价值。Survivin和VEGF在溃疡型胃癌的发生发展中起协同作用,动态随访二者对胃溃疡的演变可能有一定的价值,可作为判断肿瘤进程和浸润转移的生物学指标。Survivin及VEGF的联合检测可能对胃癌的综合治疗提供理论依据,对其进行深入研究有望为胃癌的诊疗开辟新的天地。

Expression and Investigation of Survivin and VEGF in Benign and Malignant Gastric Ulcer*

Objective： This study aimed to investigate the expression of Survivin and VEGF in benign gastric ulcer, malignant gastric ulcer, as well as the correlation between the expression in ulcerative gastric cancer and clinicopathological characteristics, and to analyze their roles in the development of gastric cancer and the relationship of expression in gastric cancer. Methods： S-P Immunohistochemical staining examinations were performed to detect different expressions of Survivin and VEGF in benign gastric ulcer, gastric ulcer with moderate to severe dysplasia and ulcerative gastric cancer. Combining with correlative clinicopathological characteristics, subsequent experimental data analysis was carried out. Results： Expression rates of Survivin and VEGF, which are significantly different （P〈0.01）, are respectively 16.2 % and 24.3 % in benign gastric ulcers, 52.3 % and 45.5 % in gastric ulcer with moderate to severe dysplasia,71.4 % and 55.3 % in ulcerative gastric cancer. Different expressions of Survivin and VEGF are related to the depth of infiltration, lymphatic metastasis and TNM staging, In addition, the expression of Survivin has positive correlations with that of VEGF. Conclusions： Survivin in ulcerative gastric cancer is highly expressed, which are the key of gastric cancer progression and indicates poor prognosis. Survivin has potential application value in the diagnosis and prognosis of gastric cancer. It may be valuable to take dynamic expression monitoring of Survivin and VEGF in gastric ulcers, for Survivin and VEGF have synergistic action in the development and progression of ulcerative gastric cancer. Survivin and VEGF can be considered indices for invasion, metastasis and progression. Furthermore, combined detection of Survivin and VEGF may provide a new theoretical basis for comprehensive reatment of gastric cancer. Further research is expected to create a new era for the diagnosis and treatment of gastric cancer.