Plant growth-promoting rhizobacteria for improving nodulation and nitrogen fixation in the common bean (Phaseolus vulgaris L.) |
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Authors: | M V B Figueiredo C R Martinez H A Burity C P Chanway |
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Institution: | (1) Laboratório de Biologia de Solo, IPA/EMBRAPA, CEP 50761-000 Recife, PE, Brazil;(2) Rua Sá e Souza, 1020/1401, CEP 51030-065 Boa Viagem, Recife, PE, Brazil;(3) Departamento de Biologia Molecular, Universidade Federal da Paraíba, Joao Pessoa, PB, Brazil;(4) Department of Forest Sciences, UBC, 3041-2424 Main Mall, Vancouver, Canada, V6T 1Z4 |
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Abstract: | A greenhouse experiment was performed to evaluate the effects of plant growth-promoting rhizobacteria (PGPR) on nodulation,
biological nitrogen fixation (BNF) and growth of the common bean (Phaseolus vulgaris L. cv. Tenderlake). Single and dual inoculation treatments of bean with Rhizobium and/or PGPR were administered to detect possible changes in the levels of and interactions between the phytohormones IAA
and cytokinin. Bean plants cv. Tenderlake were grown in pots containing Fluvic Neosol eutrophic (pH 6.5). Fourteen kilogram
aliquots of soil contained in 15-l pots were autoclaved. Bean seeds were surface sterilized and inoculated with Rhizobium tropici (CIAT 899-standard strain) alone and in combination with one of the PGPR strains: Bacillus endophyticus (DSM 13796), B. pumilus (DSM 27), B. subtilis (DSM 704), Paenibacillus lautus (DSM 13411), P. macerans (DSM 24), P. polymyxa (DSM 36), P. polymyxa (Loutit L.) or Bacillus sp.(65E180). The experimental design was randomized block design with three replications. Beans co-inoculated with Rhizobium
tropici (CIAT899) and Paenibacillus
polymyxa (DSM 36) had higher leghemoglobin concentrations, nitrogenase activity and N2 fixation efficiency and thereby formed associations of greater symbiotic efficiency. Inoculation with Rhizobium and P. polymyxa strain Loutit (L) stimulated nodulation as well as nitrogen fixation. PGPR also stimulated specific-nodulation (number of
nodules per gram of root dry weight) increases that translated into higher levels of accumulated nitrogen. The activities
of phytohormones depended on their content and interactions with Rhizobium tropici and Paenibacillus and/or Bacillus (PGPR) strains which affect the cytokinin in content in the common bean. |
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Keywords: | Symbiosis PGPR Rhizobium sp Paenibacillus polymyxa Phytohormones |
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