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1.
L. Muscatine C. Ferrier-Pagès A. Blackburn R. D. Gates G. Baghdasarian D. Allemand 《Coral reefs (Online)》1998,17(4):329-337
Symbiotic dinoflagellates are abundant in the endoderm cells of tropical marine anthozoans, but the cell-specific density
(CSD) of symbionts has not yet been investigated. In this study we used mechanical and enzymatic methods of maceration, and
staining with substrate-specific fluorochromes, to observe a large number of individual host cells from 33 species of tropical
anthozoans collected in Florida, Hawaii and Jamaica or cultured in Monaco. In the majority of species, most of the host cells
contained a single algal cell (singlet). Host cells with two or more (up to six) algae were much less abundant. The average
CSD for the 33 species was 1.54±0.30 (range 1.11 to 2.19). Singlets arranged in a monolayer can account for the areal density
of algae observed in many anthozoans. The dinoflagellates occupy most of the interior of macerated host cells, leaving the
host cytoplasm and cell membrane as a thin outer layer, often unresolvable by light microscopy. This spatial arrangement may
favor diffusion and transport of CO2, bicarbonate ions, and nutrients from the environment to the algae. The effect of nutrient enrichment on CSD was determined
by exposing eleven species to chronically elevated levels of ammonium-N. After four weeks all species exhibited a dramatic
increase in algal mitotic index and CSD. The potential consequences of environmentally induced increases in CSD in tropical
anthozoans are discussed in terms of the decreased cell-specific photosynthesis (CO2 limitation) and decreased rates of calcification observed in other studies.
Accepted: 16 February 1998 相似文献
2.
《Fungal biology》2014,118(12):943-955
The class Archaeorhizomycetes (Taphrinomycotina, Ascomycota) was introduced to accommodate an ancient lineage of soil-inhabiting fungi found in association with plant roots. Based on environmental sequencing data Archaeorhizomycetes may comprise a significant proportion of the total fungal community in soils. Yet the only species described and cultivated in this class is Archaeorhizomyces finlayi. In this paper, we describe a second species from a pure culture, Archaeorhizomyces borealis NS99-600T (=CBS138755ExT) based on morphological, physiological, and multi-locus molecular characterization. Archaeorhizomyces borealis was isolated from a root tip of a Pinus sylvestris seedling grown in a forest nursery in Lithuania. Analysis of Archaeorhizomycete species from environmental samples shows that it has a Eurasian distribution and is the most commonly observed species. Archaeorhizomyces borealis shows slow growth in culture and forms yellowish creamy colonies, characteristics that distinguish A. borealis from its closest relative A. finlayi. Here we also propose a sequence-based taxonomic classification of Archaeorhizomycetes and predict that approximately 500 species in this class remain to be isolated and described. 相似文献
3.
Glutathione (GSH) is a major antioxidant molecule in plants. It is involved in regulating plant development and responses to the abiotic and biotic environment. In recent years, numerous reports have clarified the molecular processes involving GSH in plant–microbe interactions. In this review, we summarize recent studies, highlighting the roles of GSH in interactions between plants and microbes, whether pathogenic or beneficial to plants. 相似文献
4.
Although mycosporine-like amino acids (MAAs) have been extensively investigated in reef-building corals, the sources of these
MAAs and the process of their interconversion remain a topic of interest. Here we examined ontogenetic change in the abundance
of MAAs in planula larvae of the spawning scleractinian coral Goniastrea retiformis in the absence of zooxanthellae and other dietary input. In order to examine the potential contribution of prokaryotes in
the synthesis of MAAs in animal tissue, one group of larvae were treated with the antibiotic rifampicin. High concentrations
of MAAs (mycosporine-glycine, shinorine, palythinol, asterina-330), were present in the asymbiotic eggs and adults; however,
no MAAs were present in the endosymbiotic zooxanthellae. We documented a steady decline in the total MAA concentrations through
time in larvae treated with rifampicin; however, in the absence of antibiotic there was a significant increase in the concentration
of MAAs, driven by a sharp increase in the abundance of shinorine and palythinol between day 3 and 7. Our results suggest
that MAA synthesis and conversion in G. retiformis larvae occurred in the absence of symbiotic zooxanthellae, and indicate a possible contribution of prokaryotes associated
with the animal tissue to these processes. 相似文献
5.
Daniel Wibberg Jochen Blom Christian Rückert Anika Winkler Andreas Albersmeier Alfred Pühler Andreas Schlüter Birgit E. Scharf 《Journal of biotechnology》2013
Sinorhizobium meliloti of the order Rhizobiales is a symbiotic nitrogen-fixing bacterium nodulating plants of the genera Medicago, Trigonella and Melilotus and hence is of great agricultural importance. In its free-living state it is motile and capable of modulating its movement patterns in response to chemical attractants. Here, the draft genome consisting of a circular chromosome, the megaplasmids pSymA and pSymB and three accessory plasmids of Sinorhizobium meliloti RU11/001, a model organism for flagellum structure, motility and chemotaxis, is reported. 相似文献
6.
David Durán L. Rey C. Sánchez-Cañizares A. Navarro J. Imperial T. Ruiz-Argueso 《Systematic and applied microbiology》2013
The genomic diversity of a collection of 103 indigenous rhizobia isolates from Lupinus mariae-josephae (Lmj), a recently described Lupinus species endemic to alkaline-limed soils from a restricted habitat in Eastern Spain, was investigated by molecular methods. Isolates were obtained from soils of four geographic locations in the Valencia province that harbored the known Lmj plant populations. Using an M13 RAPD fingerprinting technique, 19 distinct RAPD profiles were identified. Phylogenetic analysis based on 16S rDNA and the housekeeping genes glnII, recA and atpD showed a high diversity of native Bradyrhizobium strains that were able to establish symbiosis with Lmj. All the strains grouped in a clade unrelated to strains of the B. canariense and B. japonicum lineages that establish symbioses with lupines in acid soils of the Mediterranean area. The phylogenetic tree based on concatenated glnII, recA and atpD gene sequences grouped the Lmj isolates in six different operational taxonomic units (OTUs) at the 93% similarity level. These OTUs were not associated to any specific geographical location, and their observed divergence predicted the existence of different Bradyrhizobium genomic species. In contrast, phylogenetic analysis of symbiotic genes based on nodC and nodA gene sequences, defined only two distinct clusters among the Lmj strains. These two Lmj nod gene types were largely distinct from nod genes of bradyrhizobia nodulating other Old World lupine species. The singularity and large diversity of these strains in such a small geographical area makes this an attractive system for studying the evolution and adaptation of the rhizobial symbiont to the plant host. 相似文献
7.
Feeding by host hermit crabs Dardanus pedunculatus on their symbiotic sea anemones Calliactis polypus was investigated using animals collected at Shirahama, Wakayama Prefecture, Japan. In the first experiment, changes in the number of sea anemones on hermit crab shells were recorded in single‐and double‐crab trials without food and single‐crab trials with food. The number of sea anemones significantly decreased under starved conditions. The extent of this decrease per single hermit crab was higher in the double‐crab trials than in the single‐crab trials. Direct observations and video recordings showed that hermit crabs occasionally removed sea anemones from their own shells, and also from partners’ shells in the double‐crab trials, and consumed them. In the second experiment, fed and unfed hermit crabs with or without sea anemones were examined for body weight changes. Fed hermit crabs gained weight whereas unfed hermit crabs lost it. The degree of weight loss in unfed hermit crabs was significantly higher in those without sea anemones, which indicates some value of the latter as food. We offer some speculations on the course of development of this symbiosis, with predation on sea anemones having played an important initial role. 相似文献
8.
Blending disciplines can be transformative in science, yet interdisciplinary mergers should not escape healthy skepticism. Indeed, the history of biology shows us that debates about the relative importance of nuclear genetics vs. microbial symbiosis in eukaryotic biology are among the most engaging. Today's technology may help resolve this century old debate as it illuminates the interwoven genomics and functions of symbionts with their host genome. Thus, we can now assert that all subdisciplines of zoology require microbiology. Although controversial to some, the evidence from studies of host-associated microbial communities indicates that metazoans are hologenomes – interconnected compositions of animal and microbes. 相似文献
9.
Magriet A. van der Nest Emma T. Steenkamp Markus P. Wilken Jan Stenlid Mike J. Wingfield Brenda D. Wingfield Bernard Slippers 《Fungal biology》2013,117(6):439-450
Mutualism between microbes and insects is common and alignment of the reproductive interests of microbial symbionts with this lifestyle typically involves clonal reproduction and vertical transmission by insect partners. Here the Amylostereum fungus–Sirex woodwasp mutualism was used to consider whether their prolonged association and predominance of asexuality have affected the mating system of the fungal partner. Nucleotide information for the pheromone receptor gene rab1, as well as the translation elongation factor 1α gene and ribosomal RNA internal transcribed spacer region were utilized. The identification of rab1 alleles in Amylostereum chailletii and Amylostereum areolatum populations revealed that this gene is more polymorphic than the other two regions, although the diversity of all three regions was lower than what has been observed in free-living Agaricomycetes. Our data suggest that suppressed recombination might be implicated in the diversification of rab1, while no evidence of balancing selection was detected. We also detected positive selection at only two codons, suggesting that purifying selection is important for the evolution of rab1. The symbiotic relationship with their insect partners has therefore influenced the diversity of this gene and influenced the manner in which selection drives and maintains this diversity in A. areolatum and A. chailletii. 相似文献
10.
Simon K. Davy Karen J.T. Withers Rosalind Hinde 《Journal of experimental marine biology and ecology》2006,335(2):256-265
The nitrogen status of endosymbiotic dinoflagellates (zooxanthellae) in the temperate coral Plesiastrea versipora (Lamarck) was determined by measuring the extent to which ammonium (40 μM NH4+) enhanced the rate of zooxanthellar dark carbon fixation above that seen in filtered seawater (FSW) alone; the enhancement ratio was expressed as [dark NH4+ rate/dark FSW rate]. VD′/VL, a further index of nitrogen status, was also calculated where VD′ = [dark NH4+ rate − dark FSW rate] and VL = rate of carbon fixation in the light. When corals were starved for 2-8 weeks, zooxanthellar nitrogen deficiency became apparent at ≥ 4 weeks, with NH4+/FSW and VD′/VL averaging up to 2.08 and 0.0061, respectively. A decrease in light-saturated photosynthesis per zooxanthella also occurred, with the photosynthetic rate after 4-6 weeks being just 81% of that seen prior to starvation. In comparison, when corals were fed 5 times per week for 8 weeks the addition of ammonium had little effect, indicating nitrogen sufficiency; NH4+/FSW and VD′/VL were 1.03 and 0.0003, respectively. Photosynthetic rates of zooxanthellae from well-fed corals were up to 1.7 times greater than those of zooxanthellae from starved corals. The nitrogen status of zooxanthellae from corals in the field exhibited seasonal differences. Autumn samples were nitrogen sufficient, with NH4+/FSW = 1.003 and VD′/VL = 0.0001. In contrast, a small degree of nitrogen deficiency was seen in winter and spring, when NH4+/FSW averaged 1.075 and 1.249, and VD′/VL averaged 0.0013 and 0.0014, respectively. The greatest degree of nitrogen deficiency was observed in summer, when NH4+/FSW averaged 1.318 and VD′/VL averaged 0.0036. Given the clear links between food supply and nitrogen status seen under experimental conditions, and the likelihood that the zooxanthellae are also able to take up nutrients directly from the seawater, the fluctuations in nitrogen status may reflect temporal fluctuations in seawater nutrient concentrations and plankton abundance. The nutrient status of these temperate zooxanthellae in the field is in contrast to the marked nitrogen deficiency seen in zooxanthellae from nutrient-poor coral reef waters, and raises the possibility that temperate zooxanthellae can store nitrogen for use when exogenous nutrients and food are less readily available. This, in turn, may contribute to the considerable stability of temperate zooxanthellar populations under highly variable environmental conditions. 相似文献