Lack of a direct nitrate-trifoliin A interaction in the Rhizobium-clover symbiosis |
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Authors: | Frank B Dazzo Estelle M Hrabak |
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Institution: | (1) Department of Microbiology and Public Health, Michigan State University, 48824 East Lansing, Michigan, USA |
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Abstract: | Summary Nitrate added at critical concentrations to plant growth medium inhibits the infection of legume roots by Rhizobium. The direct
interaction, of nitrate and trifoliin A, a Rhizobium-recognition lection from white clover (Trifolium repens L.), was examined as a possible basis for this regulation. Selective molecular ultrafiltration studies to detect ligand-protein
interactions showed that radioactive13NO3
− did not bind directly to trifoliin A when incubated at two molar ratios. Immunoprecipitation of trifoliin A by its homologous
antibody was unaffected by 15 mM NO3
−. In addition, there was no apparent reduction in attachment ofR. trifolii 0403 to root hairs of clover seedings during 1 h of incubation in the presence of 15 mM NO3
−. These results show that nitrate inhibition of these early steps of the infection process is not due to a direct interaction
of nitrate with trifoliin A or its glycosylated receptors. |
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Keywords: | Clover Combined nitrogen Lectin Nitrate Nitrogen-13 Rhizobium trifolii Symbiosis Trifoliin A Trifolium repens |
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