| 基因芯片在骨骼肌老龄化相关基因表达谱中的应用 |
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| 引用本文： | 张芳林,黄国良,等.基因芯片在骨骼肌老龄化相关基因表达谱中的应用[J].生物化学与生物物理学报,2002,34(2):236-239. |
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| 作者姓名： | 张芳林 黄国良 |
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| 作者单位： | [1]上海第二医科大学附属瑞金医院，上海200025 [2]福建医科大学附属协和医院，福州350001 |
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| 基金项目： | 福建省科委三项基金资助 (No .97 Z 5 4)~~ |
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| 摘 要： | 对老龄组大鼠 (30月龄 )和年轻对照组大鼠 (3月龄 )的腓肠肌超微结构进行观察 ,可以看到前者肌肉肌纤维萎缩伴有线粒体空泡变性。并进行总RNA抽提、mRNA纯化、探针制备 ,应用基因芯片筛选老龄化相关基因 ,两组大鼠骨骼肌重复出现的差异表达基因 12 7个 ,下调基因涉及能量代谢、信号转导 ,上调基因涉及蛋白质分解、细胞凋亡
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| 关 键 词： | 基因芯片 基因表达谱 衰老 骨骼肌 |
Gene Expression Pattern of Senescence Associated Genes in Rat Muscle |
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| ZHANG Fang-Lin ,HUANG Guo-Liang ,YE Chuan-Zhong ,ZHANG Kai-Zhen ,LIN Zhe-Zhang ,CHEN Xiao-Chun ,YOU Jie.Gene Expression Pattern of Senescence Associated Genes in Rat Muscle[J].Acta Biochimica et Biophysica Sinica,2002,34(2):236-239. |
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| Authors: | ZHANG Fang-Lin HUANG Guo-Liang YE Chuan-Zhong ZHANG Kai-Zhen LIN Zhe-Zhang CHEN Xiao-Chun YOU Jie |
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| Institution: | ZHANG Fang-Lin *1,2,HUANG Guo-Liang 2,YE Chuan-Zhong 3,ZHANG Kai-Zhen 2,LIN Zhe-Zhang 2,CHEN Xiao-Chun 4,YOU Jie 2 |
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| Abstract: | This work is aimed at the investigation of the senescence associated genes differentially expressed in rat muscle by cDNA microarray. After total RNA isolation, mRNA purification and probe preparation, the gene expression patterns of rat muscle from the old group (30 months old) and the young group (3 months old) were compared by cDNA gene chips. In the muscle of old rats there were reproducibly 127 differentially expressed genes, among which some down-regulated genes were involved in energy metabolism and signal transduction, while some up-regulated genes were related to protein degradation and cell apoptosis. Meanwhile muscle fiber atrophy with mitochondria vacuolation was found in aging rat by electron microscope. |
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| Keywords: | gene chip gene expression pattern senescence skeletal muscle |
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