| 稻瘟病菌重复顺序PoR6和稻瘟病菌的分型 |
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| 引用本文： | 朱衡,翟文学,陈美玲,王久林,雷财林,凌忠专,朱立煌.稻瘟病菌重复顺序PoR6和稻瘟病菌的分型[J].菌物学报,1995,14(3). |
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| 作者姓名： | 朱衡 翟文学 陈美玲 王久林 雷财林 凌忠专 朱立煌 |
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| 作者单位： | 1.中国科学院遗传研究所 北京 100101；2.中国农业科学院作物研究所 北京 100081 |
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| 摘 要： | POR6是一具有高度多态性的稻瘟病菌(Pyricularia oryzae)重复顺序。利用脉冲电泳技术和Southern分析,表明它是非均匀地散布于基因组中的。经测定POR6的拷贝数约为30—40,序列测定未发现在内部有更小的重复单位。用POR6作探针对44株稻瘟病菌进行DNA指纹分析,分析的中国北方地区的22个菌株可根据相似率归并成8个谱系。对一些转管培养中致病型发生变化的菌株用POR6进行指纹分析,发现这些菌株在转管过程中基因组DNA是有变化的。
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| 关 键 词： | 稻瘟病菌 重复顺序 DNA指纹图谱 脉冲电泳 |
REPEATED SEQUENCE POR6 AND TYPING OF PYRICVLARIA ORYZAE ISOLATES |
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| Authors: | Zhu Heng Zhai Wenxue Chen Meiling Wang Jiulin Lei Cailin Ling Zhongzhuan Zhu Lihuang Institute of Genetics Academia Sinica Beijing Institute of Crop Breeding and Cultivation Chinese Academy of Agricultural Sciences Beijing |
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| Institution: | Zhu Heng Zhai Wenxue Chen Meiling Wang Jiulin Lei Cailin Ling Zhongzhuan Zhu Lihuang Institute of Genetics,Academia Sinica,Beijing 100101 Institute of Crop Breeding and Cultivation,Chinese Academy of Agricultural Sciences,Beijing 100081 |
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| Abstract: | A repeated sequence, POR6, was proved highly polymorphic in Pyricularia oryzae isolates. The results of pulse field gel electrophoresis and southern hybridization indicated that POR6 sequence was randomly dispersed in P. oryzae genomes with a 30 to 40 copy number. There was no smaller repeated unit discovered within POR6 sequence. POR6-DNA was used as a probe to fingerprint 44 P. oryzae isolates. Based on the similarity of fingerprinting, 22 isolates collected from the north China could be divided into 8 lineages. For some isolates whose infection spectrum had been changed after serial transfers in culture, their corresponding fingerprintings indicated that changes also had taken place in their genomes. |
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| Keywords: | rice blast fungus repeated sequences DNA fingerprinting pulse field gel electrophoresis |
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